Nelfb (NM_021393) Mouse Untagged Clone

CAT#: MC200028

Nelfb (untagged) - Mouse cofactor of BRCA1 (Cobra1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nelfb
• Synonyms: A730008L03Rik; AB041607; AI663983; Cob; Cobra1; Nelf-b
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC004762 sequence for NM_021393
  GAGCTGCTCTGCTGACGCGATCTGGCCACACTGGAGGCGGCGGGCGAACGGGGCTTGGGGGGTCCTCGTG GCACCGTGGAGAGGGCTTCTGGGGCTCCGTCAGGGTCCGCTACGGCTCCAGCGGAGCGCGGTGGGGATGG GGTACACAGCCGAGCGTCCGCCGGAGCCTCCGCCATGTTCGCGGGGCTCCAGGACCTGGGCGTGGCCAAT GGCGAGGACCTGAAAGAGACCCTGACCAACTGCACCGAGCCTCTCAAGGCCATTGAACAATTTCAGACAG AGAATGGCGTGTTGCTGCCTTCCCTGCAGTCGGCCCTGCCCTTCTTGGATCTACACGGGACACCGCGGCT GGAGTTCCATCAGTCAGTGTTTGATGAACTACGTGACAAACTTCTGGAACGAGTGTCAGCAATTGCCTCT GAGGGAAAGGCAGAGGAGAGGTACAAGAAGCTGGAAGACCTTCTGGAAAAGAGCTTTTCTTTGGTCAAGA TGCCATCCCTACAACCAGTGGTGATGTGTGTTATGAAACACTTGCCCAAGGTTCCTGAGAAGAAGCTGAA GCTGGTGATGGCTGACAAGGAGTTGTACCGGGCATGTGCTGTGGAAGTGAAGAGGCAGATTTGGCAAGAC AACCAGGCTCTCTTTGGTGATGAAGTCTCACCACTGCTGAAGCAGTATATCCTAGAGAAAGAGAGTGCGC TCTTCAGCACAGAGCTCTCTGTGCTGCACAACTTCTTCAGCCCTTCCCCCAAGACCCGGCGCCAAGGAGA GGTGGTGCAGAAGCTGACGCAGATGGTAGGGAAGAATGTGAAGCTGTACGACATGGTGCTGCAGTTCCTG CGCACACTGTTCCTGCGGACCAGGAATGTGCACTACTGCACGTTACGAGCTGAGCTGCTCATGTCTTTGC ATGACTTGGACGTCAGTGACATCTGCACTGTGGATCCCTGCCATAAGTTCACCTGGTGCCTGGATGCCTG CATCCGGGAACGGTTTGTGGACAGCAAAAGAGCCCGGGAGCTTCAGGGATTTCTTGATGGTGTGAAGAAG GGCCAGGAACAAGTTTTGGGGGATTTGTCTATGATCCTGTGCGACCCCTTTGCCATCAACACACTGTCCC TGAGCACCATCAGGCACTTGCAGGAGCTGGTTAGCCAGGAGACACTGCCCAGGGATAGTCCCGACCTCCT CCTACTACTTAGGCTGCTGGCACTGGGCCAAGGTGCATGGGACTTAATAGATAGCCAGGTCTTCAAGGAG CCTAAGATGGAGGCAGAGCTCATCACCAAGTTCCTGCCGATGCTCATGTCCCTCGTGGTGGATGACTTCA CTTTCAACGTAGATCAGAAGCTTCCAGCTGAGGAGAAAGCCTCAGTCACATACCCCAACACACTTCCTGA GAGTTTCACCAAGTTTCTGCAGGAGCAGCGCATGGCTTGTGAGGTGGGACTATACTACGTCCTACACATC ACCAAGCAGAGAAACAAGAATGCTCTCCTGCGCCTGCTGCCTGGACTTGTGGAAACGTTTGGTGACCTGG CCTTCAGTGACATCTTTCTCCACCTGCTCACTGGGAGCCTGGTACTGCTTGCTGATGAATTTGCCCTGGA GGATTTCTGCAGCAGCCTCTTTGATGGCTTCTTCCTCACTGCCTCTCCCAGGAAGGAGAATGTGCACCGT CATGTGCTGAGGCTTCTCCTCCACTTGCATGCAAGAGTGGCCCCTTCCAAGCTGGAGGCATTGCAGAAGG CTCTGGAGCCCACAGGCCAGAGCGGGGAGGCCGTGAAGGAGCTCTACTCCCAGCTTGGTGAGAAGTTGGA GCAGTTAGACCATCGAAAGCCCAGTCCTACCCAAGCTGCAGAGACACCAGCACTGGATCTGCCTCTTCCC AGCGTGCCTGCTCCAGCCACACTGTGAGGCTGTGGAGAACTGAAACTGTTTGACAGTGGTGCCCTATGGA AGGGCACCTAGTTGGCTAGGGGTGTAGGCTGAGCTGAGAGGGGTGTTCTTTGGCAAGGTTCTCCCTCCTC TAGTTTCTCCCAGGAGCTGGTCCCAAAAGGTCCTCCCTCAGTGGTCTACTTGCATATCATAGGGGGCCCT TGATGTCATGGTGGGACTTGGCCCCTGCTTGACCCTGGCAGAGGTGGATGGTGATTGGGATATAGGAAAA GCAGTGGGCAGAGGCCTGACTGACCTGATATTCAATCTGCACAAAGTCCAAGGTATGCTAGGTTCACCAA AGTAGATTTGCTGGACCTGATCCCCAAGCACAGGAGTGGTACCCTCCACAGAAGCTGGGGGAGAGTCTGC AGCAGGCCTGAGCCTGCTGATGTCAGTGCTGACTGAGTCCATGCTAACTGAGATCTGTAGGCAGAGCTCT GCCCTGTAGCTCCCTGCCTCAGTTTATCTTTGCAATACAAAGCAAATCCTTCCCCAGCTATGTGTTTTAG CCTTTGAGTAGGGGAGTTTCGGTGTACACATTGAATTTTCCACTTATGTAAATAAAACGGATTTTGATAA AAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_021393
• Insert Size: 1428 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004762, AAH04762
• RefSeq Size: 2533 bp
• RefSeq ORF: 1428 bp
• Locus ID: 58202
• UniProt ID: Q8C4Y3
• Cytogenetics: 2 A3
• Gene Summary: This gene encodes subunit B of a metazoan-specific, four-subunit protein complex that regulates promoter-proximal pausing of RNA polymerase II. RNA polymerase II pausing is thought to be important for coordination of gene transcription during embryonic development and stress responses. Consistently, disruption of this gene in mouse causes inner cell mass deficiency and embryonic lethality. In addition, this gene is required for maintenance of mouse embryonic stem cells by preventing expression of developmental genes. In adult mice, conditional deletion of this gene results in cardiomyopathy and impaired response to cardiac stress. Multiple protein isoforms are encoded through the use of a non-AUG (CUG) initiation codon and an alternative downstream AUG initiation codon. In addition, alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
  Transcript Variant: This variant (1) represents the longer isoform (1), which is translated using a non-AUG (CUG) start codon. CCDS Note: The coding region has been updated to extend the protein N-terminus and to use a non-AUG (CUG) start codon, supported by data in PMID:26010750.