Human SKA3 (NM_145061) AAV Particle

SKU RC222931A1V

AAV ORF Particles, serotype AAV-2, SKA3 (Myc-DDK-tagged)-Human spindle and kinetochore associated complex subunit 3 (SKA3), transcript variant 1, 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: SKA3
• Synonyms: C13orf3; RAMA1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222931 representing NM_145061; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGACCCTATCCGGAGCTTCTGCGGGAAGCTGCGGTCTCTGGCCAGCACGCTGGACTGCGAGACGGCCC; GGCTGCAGCGAGCGCTGGACGGAGAGGAAAGCGACTTTGAAGATTATCCAATGAGAATTTTATATGACCT; TCATTCAGAAGTTCAGACTCTAAAGGATGATGTTAATATTCTTCTTGATAAAGCAAGATTGGAAAATCAA; GAAGGCATTGATTTCATAAAGGCAACAAAAGTACTAATGGAAAAAAATTCAATGGATATTATGAAAATAA; GAGAGTATTTCCAGAAGTATGGATATAGTCCACGTGTCAAGAAAAATTCAGTACACGAGCAAGAAGCCAT; TAACTCTGACCCAGAGTTGTCTAATTGTGAAAATTTTCAGAAGACTGATGTGAAAGATGATCTGTCTGAT; CCTCCTGTTGCAAGCAGTTGTATTTCTGAGAAGTCTCCACGTAGTCCACAACTTTCAGATTTTGGACTTG; AGCGGTACATCGTATCCCAAGTTCTACCAAACCCTCCACAGGCAGTGAACAACTATAAGGAAGAGCCCGT; AATTGTAACCCCACCTACCAAACAATCACTAGTAAAAGTACTAAAAACTCCAAAATGTGCACTAAAAATG; GATGATTTTGAGTGTGTAACTCCTAAATTAGAACACTTTGGTATCTCTGAATATACTATGTGTTTAAATG; AAGATTACACAATGGGACTTAAAAATGCGAGGAATAATAAAAGTGAGGAGGCCATAGATACAGAATCCAG; GCTCAATGATAATGTTTTTGCCACTCCCAGCCCCATCATCCAGCAGTTGGAAAAAAGTGATGCCGAATAT; ACCAACTCTCCTTTGGTACCTACATTCTGTACTCCTGGTTTGAAAATTCCATCTACAAAGAACAGCATAG; CTTTGGTATCCACAAATTACCCATTATCAAAAACAAATAGTTCATCAAATGATTTGGAAGTTGAAGATCG; TACTTCGTTGGTTTTAAATTCAGACACATGCTTTGAGAATTTAACAGATCCCTCTTCACCTACGATTTCT; TCTTATGAGAATCTGCTCAGAACACCTACACCTCCAGAAGTAACTAAAATTCCAGAAGATATTCTCCAGC; TTTTATCAAAATACAACTCAAACCTAGCTACTCCAATAGCAATTAAAGCAGTGCCACCCAGTAAAAGGTT; CCTTAAACATGGACAGAACATCCGAGATGTCAGCAACAAAGAAAAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222931 representing NM_145061; Red=Cloning site Green=Tags(s); ; MDPIRSFCGKLRSLASTLDCETARLQRALDGEESDFEDYPMRILYDLHSEVQTLKDDVNILLDKARLENQ; EGIDFIKATKVLMEKNSMDIMKIREYFQKYGYSPRVKKNSVHEQEAINSDPELSNCENFQKTDVKDDLSD; PPVASSCISEKSPRSPQLSDFGLERYIVSQVLPNPPQAVNNYKEEPVIVTPPTKQSLVKVLKTPKCALKM; DDFECVTPKLEHFGISEYTMCLNEDYTMGLKNARNNKSEEAIDTESRLNDNVFATPSPIIQQLEKSDAEY; TNSPLVPTFCTPGLKIPSTKNSIALVSTNYPLSKTNSSSNDLEVEDRTSLVLNSDTCFENLTDPSSPTIS; SYENLLRTPTPPEVTKIPEDILQLLSKYNSNLATPIAIKAVPPSKRFLKHGQNIRDVSNKEN; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_145061
• ORF Size: 1236 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_145061.3, NP_659498.2
• RefSeq Size: 2907 bp
• RefSeq ORF: 1239 bp
• Locus ID: 221150
• UniProt ID: Q8IX90
• Cytogenetics: 13q12.11
• MW: 46.2 kDa