Human PRAF1 (POLR1E) (NM_022490) AAV Particle

SKU RC204811A1V

AAV ORF Particles, serotype AAV-2, POLR1E (Myc-DDK-tagged)-Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E), 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: PRAF1
• Synonyms: A49; PAF53; PRAF1; RPA49
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204811 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGCGGAGGTGTTGCCGAGTGCGAGGTGGCAGTATTGTGGGGCGCCCGACGGGAGCCAGAGAGCTG; TACTGGTCCAGTTCTCCAACGGGAAGCTACAGAGTCCAGGCAACATGCGCTTTACCTTGTATGAGAACAA; AGATTCCACCAACCCCAGGAAGAGGAATCAACGGATCCTGGCAGCTGAAACAGATAGGCTCTCCTATGTG; GGAAACAATTTTGGGACTGGAGCCCTCAAATGCAACACTTTGTGCAGGCACTTTGTGGGAATTTTGAACA; AGACCTCTGGCCAAATGGAAGTATATGATGCTGAATTGTTCAACATGCAGCCACTATTTTCAGATGTATC; AGTTGAGAGTGAACTGGCTCTAGAGAGTCAGACCAAAACTTACAGAGAAAAGATGGATTCTTGTATTGAA; GCCTTTGGTACCACTAAACAGAAGCGAGCTCTGAACACCAGGAGAATGAACAGAGTTGGCAATGAATCTT; TGAATCGTGCAGTGGCTAAAGCTGCAGAGACTATCATTGATACGAAGGGTGTGACTGCTCTGGTCAGCGA; TGCTATCCACAATGACTTGCAAGATGACTCCCTCTACCTTCCTCCCTGCTATGATGATGCAGCCAAGCCT; GAAGACGTGTATAAATTTGAAGATCTTCTTTCCCCTGCGGAGTATGAAGCTCTTCAGAGCCCATCTGAAG; CTTTCAGGAACGTCACGTCAGAAGAAATACTGAAGATGATTGAGGAGAACAGCCATTGCACCTTTGTCAT; AGAAGCGTTGAAGTCTTTGCCATCAGATGTGGAGAGCCGAGACCGCCAGGCCCGATGCATATGGTTTCTG; GATACCCTCATCAAATTTCGAGCTCATAGGGTAGTTAAGCGGAAAAGTGCTCTGGGACCTGGAGTTCCCC; ACATCATCAACACCAAACTGCTGAAGCACTTTACCTGCTTGACCTACAACAATGGCAGATTACGGAACTT; AATTTCGGATTCTATGAAGGCGAAGATTACTGCATATGTGATCATACTTGCCTTGCACATACATGACTTC; CAAATTGACCTGACAGTGTTACAGAGGGACTTGAAGCTCAGTGAGAAAAGGATGATGGAGATAGCCAAAG; CCATGAGGCTGAAGATCTCCAAAAGAAAGGTGTCTGTGGCCGCCGGCAGTGAAGAAGATCACAAACTGGG; CACCCTGTCCCTCCCGCTGCCTCCAGCCCAGACCTCAGACCGCCTGGCAAAGCGGAGGAAGATTACC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204811 protein sequence; Red=Cloning site Green=Tags(s); ; MAAEVLPSARWQYCGAPDGSQRAVLVQFSNGKLQSPGNMRFTLYENKDSTNPRKRNQRILAAETDRLSYV; GNNFGTGALKCNTLCRHFVGILNKTSGQMEVYDAELFNMQPLFSDVSVESELALESQTKTYREKMDSCIE; AFGTTKQKRALNTRRMNRVGNESLNRAVAKAAETIIDTKGVTALVSDAIHNDLQDDSLYLPPCYDDAAKP; EDVYKFEDLLSPAEYEALQSPSEAFRNVTSEEILKMIEENSHCTFVIEALKSLPSDVESRDRQARCIWFL; DTLIKFRAHRVVKRKSALGPGVPHIINTKLLKHFTCLTYNNGRLRNLISDSMKAKITAYVIILALHIHDF; QIDLTVLQRDLKLSEKRMMEIAKAMRLKISKRKVSVAAGSEEDHKLGTLSLPLPPAQTSDRLAKRRKIT; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_022490
• ORF Size: 1257 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_022490.1
• RefSeq Size: 1884 bp
• RefSeq ORF: 1260 bp
• Locus ID: 64425
• UniProt ID: Q9GZS1
• Cytogenetics: 9p13.2
• MW: 47.2 kDa