ABCA1 (NM_005502) Human 3' UTR Clone

CAT#: SC220652

3' UTR clone of ATP-binding cassette sub-family A (ABC1) member 1 (ABCA1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ABCA1
• Synonyms: ABC-1; ABC1; CERP; HDLCQTL13; HDLDT1; HPALP1; TGD
• ACCN: NM_005502
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC220652 3’UTR clone of NM_005502
  The sequence shown below is from the reference sequence of NM_005502. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GATGAGAAAGTGAAAGAAAGCTATGTATGAAGAATCCTGTTCATACGGGGTGGCTGAAAGTAAAGAGGA
  ACTAGACTTTCCTTTGCACCATGTGAAGTGTTGTGGAGAAAAGAGCCAGAAGTTGATGTGGGAAGAAGT
  AAACTGGATACTGTACTGATACTATTCAATGCAATGCAATTCAATGCAATGAAAACAAAATTCCATTAC
  AGGGGCAGTGCCTTTGTAGCCTATGTCTTGTATGGCTCTCAAGTGAAAGACTTGAATTTAGTTTTTTAC
  CTATACCTATGTGAAACTCTATTATGGAACCCAATGGACATATGGGTTTGAACTCACACTTTTTTTTTT
  TTTTTTGTTCCTGTGTATTCTCATTGGGGTTGCAACAATAATTCATCAAGTAATCATGGCCAGCGATTA
  TTGATCAAAATCAAAAGGTAATGCACATCCTCATTCACTAAGCCATGCCATGCCCAGGAGACTGGTTTC
  CCGGTGACACATCCATTGCTGGCAATGAGTGTGCCAGAGTTATTAGTGCCAAGTTTTTCAGAAAGTTTG
  AAGCACCATGGTGTGTCATGCTCACTTTTGTGAAAGCTGCTCTGCTCAGAGTCTATCAACATTGAATAT
  CAGTTGACAGAATGGTGCCATGCGTGGCTAACATCCTGCTTTGATTCCCTCTGATAAGCTGTTCTGGTG
  GCAGTAACATGCAACAAAAATGTGGGTGTCTCCAGGCACGGGAAACTTGGTTCCATTGTTATATTGTCC
  TATGCTTCGAGCCATGGGTCTACAGGGTCATCCTTATGAGACTCTTAAATATACTTAGATCCTGGTAAG
  AGGCAAAGAATCAACAGCCAAACTGCTGGGGCTGCAAGCTGCTGAAGCCAGGGCATGGGATTAAAGAGA
  TTGTGCGTTCAAACCTAGGGAAGCCTGTGCCCATTTGTCCTGACTGTCTGCTAACATGGTACACTGCAT
  CTCAAGATGTTTATCTGACACAAGTGTATTATTTCTGGCTTTTTGAATTAATCTAGAAAATGAAAAGAT
  GGAGTTGTATTTTGACAAAAATGTTTGTACTTTTTAATGTTATTTGGAATTTTAAGTTCTATCAGTGAC
  TTCTGAATCCTTAGAATGGCCTCTTTGTAGAACCCTGTGGTATAGAGGAGTATGGCCACTGCCCCACTA
  TTTTTATTTTCTTATGTAAGTTTGCATATCAGTCATGACTAGTGCCTAGAAAGCAATGTGATGGTCAGG
  ATCTCATGACATTATATTTGAGTTTCTTTCAGATCATTTAGGATACTCTTAATCTCACTTCATCAATCA
  AATATTTTTTGAGTGTATGCTGTAGCTGAAAGAGTATGTACGTACGTATAAGACTAGAGAGATATTAAG
  TCTCAGTACACTTCCTGTGCCATGTTATTCAGCTCACTGGTTTACAAATATAGGTTGTCTTGTGGTTGT
  AGGAGCCCACTGTAACAATACTGGGCAGCCTTTTTTTTTTTTTTTTTAATTGCAACAATGCAAAAGCCA
  AGAAAGTATAAGGGTCACAAGTCTAAACAATGAATTCTTCAACAGGGAAAACAGCTAGCTTGAAAACTT
  GCTGAAAAACACAACTTGTGTTTATGGCATTTAGTACCTTCAAATAATTGGCTTTGCAGATATTGGATA
  CCCCATTAAATCTGACAGTCTCAAATTTTTCATCTCTTCAATCACTAGTCAAGAAAAATATAAAAACAA
  CAAATACTTCCATATGGAGCATTTTTCAGAGTTTTCTAACCCAGTCTTATTTTTCTAGTCAGTAAACAT
  TTGTAAAAATACTGTTTCACTAATACTTACTGTTAACTGTCTTGAGAGAAAAGAAAAATATGAGAGAAC
  TATTGTTTGGGGAAGTTCAAGTGATCTTTCAATATCATTACTAACTTCTTCCACTTTTTCCAGAATTTG
  AATATTAACGCTAAAGGTGTAAGACTTCAGATTTCAAATTAATCTTTCTATATTTTTTAAATTTACAG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005502.4
• Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in both alleles of this gene cause Tangier disease and familial high-density lipoprotein (HDL) deficiency. [provided by RefSeq, Sep 2019]
• Locus ID: 19
• MW: 76.7