FADS2 (NM_004265) Human 3' UTR Clone

CAT#: SC215878

3' UTR clone of fatty acid desaturase 2 (FADS2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: FADS2
• Synonyms: D6D; DES6; FADSD6; LLCDL2; SLL0262; TU13
• ACCN: NM_004265
• Insert Size: 1695 bp
• Sequence Data:
  Insert Sequence

  >SC215878 3’UTR clone of NM_004265
  The sequence shown below is from the reference sequence of NM_004265. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTGTGGCTGGACGCCTACCTTCACAAATGAAGCCACAGCCCCCGGGACACCGTGGGGAAGGGGTGCAGG
  TGGGGTGATGGCCAGAGGAATGATGGGCTTTTGTTCTGAGGGGTGTCCGAGAGGCTGGTGTATGCACTG
  CTCACGGACCCCATGTTGGATCTTTCTCCCTTTCTCCTCTCCTTTTTCTCTTCACATCTCCCCCATAGC
  ACCCTGCCCTCATGGGACCTGCCCTCCCTCAGCCGTCAGCCATCAGCCATGGCCCTCCCAGTGCCTCCT
  AGCCCCTTCTTCCAAGGAGCAGAGAGGTGGCCACCGGGGGTGGCTCTGTCCTACCTCCACTCTCTGCCC
  CTAAAGATGGGAGGAGACCAGCGGTCCATGGGTCTGGCCTGTGAGTCTCCCCTTGCAGCCTGGTCACTA
  GGCATCACCCCCGCTTTGGTTCTTCAGATGCTCTTGGGGTTCATAGGGGCAGGTCCTAGTCGGGCAGGG
  CCCCTGACCCTCCCGGCCTGGCTTCACTCTCCCTGACGGCTGCCATTGGTCCACCCTTTCATAGAGAGG
  CCTGCTTTGTTACAAAGCTCGGGTCTCCCTCCTGCAGCTCGGTTAAGTACCCGAGGCCTCTCTTAAGAT
  GTCCAGGGCCCCAGGCCCGCGGGCACAGCCAGCCCAAACCTTGGGCCCTGGAAGAGTCCTCCACCCCAT
  CACTAGAGTGCTCTGACCCTGGGCTTTCACGGGCCCCATTCCACCGCCTCCCCAACTTGAGCCTGTGAC
  CTTGGGACCAAAGGGGGAGTCCCTCGTCTCTTGTGACTCAGCAGAGGCAGTGGCCACGTTCAGGGAGGG
  GCCGGCTGGCCTGGAGGCTCAGCCCACCCTCCAGCTTTTCCTCAGGGTGTCCTGAGGTCCAAGATTCTG
  GAGCAATCTGACCCTTCTCCAAAGGCTCTGTTATCAGCTGGGCAGTGCCAGCCAATCCCTGGCCATTTG
  GCCCCAGGGGACGTGGGCCCTGCAGGCTGCAGGAGGGCACTGGAGCTGGGAGGTCTCGTCCCAGCCCTC
  CCCATCTCGGGGCTGCTGTGTGGACGGCGCTGCCTCAGGCACTCTCCTGTCTGAACCTGCCCTTACTGT
  GTTTAACCTGTTGCTCCAGGATGCATTCTGATAGGAGGGGGCGGCAGGGCTGGGCCTTGTGACAATCTG
  CCTTTCACCACATGGCCTTGCCTCGGTGGCCCTGACTGTCAGGGAGGGCCAGGGAGGCAGAGCGGGAGG
  GAGTCTCAGGAGGAGGCTGCCCTGAGGGGCTGGGGAGGGGGTACCTCATGAGGACCAGGGTGGAGCTGA
  GAAGAGGAGGAGGTGGGGGCTGGAGGTGCTGGTAGCTGAGGGGACGGGCAAGTGAGAGGGGAGGGAGGG
  AAGTCCTGGGAGGATCCTGAGCTGCTGTTGCAGTCTAACCCACTAATCAGTTCTTAGATTCAGGGGAAG
  GGCAGGCACCAACAACTCAGAATGGGGGCTTTCGGGGAGGGCGCCTAGTCCCCCCAGCTCTAAGCAGCC
  AGGAGGGACCTGCATCTAAGCATCTGGGTTGCCATGGCAATGGCATGCCCCCCAGCTACTGTATGCCCC
  CGACCCCCGCAGAGGCAGAATGAACCCATAGGGAGCTGATCGTAATGTTTATCATGTTACTTCCCCACC
  CCTACATTTTTTGAAATAAAATAAGGAATTTTATTCTCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004265.4
• Summary: The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
• Locus ID: 9415
• MW: 59.3