IKAP (IKBKAP) (NM_003640) Human 3' UTR Clone

CAT#: SC215602

3' UTR clone of inhibitor of kappa light polypeptide gene enhancer in B-cells kinase complex-associated protein (IKBKAP) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ELP1
• Synonyms: DYS; FD; IKAP; IKBKAP; IKI3; TOT1
• ACCN: NM_003640
• Insert Size: 1628 bp
• Sequence Data:
  Insert Sequence

  >SC215602 3’UTR clone of NM_003640
  The sequence shown below is from the reference sequence of NM_003640. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ACCCAGTGGAAGCTGAGCCTGCTAGACTGAGTGACTGCAGTTAGGAGGGATCCGACAGAGAAGACCATT
  TCCACTCATTCCTGTTGTCCTACCACCCCTTGCTCTTTGAGGGCTGGCTATTGAGAACTGGAAAGAGTA
  AAATGATAACTTACCTTAGCATTGCCAAGAACTTCAGCAGACAACAAGCAATTCTATTTATTTTATGTT
  GTGTATACATCTTGATCATTAGCAAGACATTAAGCTTTAACCATTATGGCACCATTTTGTGAGAATGAT
  TGTTCTTTCACTTGGGCTGTTTGAGAGCATAATTATGGTAATCATGAGATTAATGTTTCATGATTTCTA
  CCTCCAAAGTGTGAAGACAAGTAAAACAATGTTTCTAAATTGTCTTATTTTGTTGGCGGAGAAGATTAC
  AATGGCTATTAGTGCTACATTTGGTCAAATGTAATCACTTAAATAGCTTCTTGTCACCTTAAACTAAAG
  CAGAATAAAAAGTATCCTTTGAAATTATAAGCCCTCCTTTGCTGACAGCTATTATTTTGTAACATCTTA
  CCAGGTCATGTGCTTTCAGTTATAACTGGGCTGAGCCTCCTATAATTACAATGTCTATAGGGACTGTTT
  TACTGCCTGTGTATTTTCTGCTAGAGAGTTAGCAATGTTAGAGCTAGAACAGATTAGAATTTCTAAACA
  GTATCATGCACAGTTGGTGTGAGTGATCAGTGTGCATTGTATGGCATGCATGGTTGTGAATTATTCTCT
  GTTCTCCAAATACTGTTTCTTTAACTCAGATATTTTTGTTAGTGTCTAGGCCACTTCATTTATTTTTCG
  TCATGGTACTTTACTGACTTCTCTTTATTCAATTCTCCACGCCCTCACCAAAAAAAACTGTCTCAAAAT
  GAGAATATTTTATTTTCATGGTGAGTCTAGAAAACGCCCACTTCATTCTGATTAAAAATTCTTCCATGT
  TTTAAATATCAGAACCAGACCTTTCTTACTGTGTATCTTAGCCCATTTGTGTCTCTATAACAACAACCA
  GCTTTCAAAGGAACTAATAGAGTGAAAACTCACTCATTACCACGAGGATGGCACAAGCGATTCACGTAG
  GATCTGCCCCTGTGACCAAAACACCTCCCATTGGGCCCCACTTCCAACACTGGTGATCACATTTCAACA
  TGAGGTTTAGGGAAACAAATGCCTAAACTACAGCACTGTACATAAACTAACAGGAAATGCTGCTTTTGA
  TCCTCAAAGAAGTGATATAGCCAAAATTGTAATTTAAGAAGCCTTTCCCAGTATAGCAAGATGTTAACT
  ATAGAATCAATCTAGGAGTATTCACTGTAAAATTCAACTTTTCTGTATGTTTGAACATTTTCACAATCT
  CATAGGAGTTTTTAAAAAGAAGAGAAAGAAGATATACTTTGCTTTGGAGAAATCTACTTTTTGACTTAC
  ATGGGTTTGCTGTAATTAAGTGCCCAATATTGAAAGGCTGCAAGTACTTTGTAATCACTCTTTGGCATG
  GGTAAATAAGCATGGTAACTTATATTGAAATATAGTGCTCTTGCTTTGGATAACTGTAAAGGGACCCAT
  GCTGATAGACTGGAAATAGAAGTAAATGTGTTTATTGATAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003640.5
• Summary: The protein encoded by this gene is a scaffold protein and a regulator for three different kinases involved in proinflammatory signaling. The encoded protein can bind NF-kappa-B-inducing kinase and I-kappa-B kinases through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
• Locus ID: 8518
• MW: 62.1