PTPN20A (NM_001042390) Human 3' UTR Clone

CAT#: SC215425

3' UTR clone of protein tyrosine phosphatase non-receptor type 20A (PTPN20A) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PTPN20A
• Synonyms: bA142I17.1; CT126; hPTPN20; PTPN20B
• ACCN: NM_001042390
• Insert Size: 1609 bp
• Sequence Data:
  Insert Sequence

  >SC215425 3’UTR clone of NM_001042390
  The sequence shown below is from the reference sequence of NM_001042390. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GTTCTTCGGAAACTTCTGACTTTGGATTAAGAAAGACTTCTGTTGCCTCTCACTTGAAATTACCAAGTG
  GGTTTGCACCTCCTCATAAAGAACATGTTTGCACTGTGCTGAAGGGCTTTGCTATGCATACAATCTGCT
  TTCTTGGTTTATCAGTTTATTTTCTTTCTAAAAGCTCCCTGAAGGGCAATATCATTTGGCTTGGGGTGA
  TCAGTGTTTACTTATTGATCTTGCTAGACAATATCAAAATAACTTCCCACATTTTCCAGTGAAACAGAT
  GTTACATAAAACGATTGCAGCTTGGCTATTTGGTTGAAGGGATTACAGAGCCCAATAAAGGATTTAAAA
  TATATTCATTAAGATTTTATTTGGAAAGGTGGCTGGAGAGAGCTGAGGATTTCCAGGACTTTGTAAGTT
  CTTATTCTGGGAGAACATAAGGCCAATAATCATGACCTCTTCCAGGCATTTTTAAGACAGATGTCTATT
  CATGTTCTTTAGCTAGAGCCTGTACTTTTTGCTGGCATTTGAATAACCCAGTTTAAAAAGAGTCCAGTT
  AGGGTGGACTAACTTTGGACACAAATTGGCTTCCATTTCCTACATTTTCATACTGCTGCCTTCCTACAG
  CTGCTAGACCAAGACCTGTTGGTCTGGGAAGCATTTCATGGATAGGGAGAGCTCCTCTCGGTGAACAGT
  CCAAAACTAAAATAGATGTTTATATAGAAAGCCCAAGAGGAGATTTTTGCCATGCCTGAGTTCTTTCCT
  ATCCCACCCTAACACTTAACATATTACTTAGTCTGCTTTGTTAAAAGCAAGTATTACCTTTAACTTGCC
  TCTTACTCTTTGCCCTTTAGCTAACTAATAAAGTTTGATATAGGCATTATTATATAATTCTGAGTCATT
  CATGGTATATCTCATGTTTGATGTATTTTTCAAACTAAGATCTATGATAGTTTTTTTTCCAGAGTTCCA
  TTAAATCATTTATTTCCTTTACTTTCTCACCTCTGTTGAAACATTTAGAAACTGGATTTGGGAACCCAA
  TTTTGGAAAACCAGATTCATAGTCATGAAAATGGAAACTTCCATATTCTGTTTTTGAAAAGATGTGGCC
  ATTATTACAGTAATTTTATTATAGGACTTTGCCTCGTACAATTAATAGTGATATTTTGGACAAGGAGTT
  CTGGTGACAAGCTATACCTAATTATAAGCTATAAAACAATAGATATGAGTGTTTGTACAGTTTAACTCA
  ATGGAGATCAGAATATTCTATGTATTGAGAAAATGTTTAATATCAATCTATAAATCTTGAATTTCTAAG
  AGGCTTATTTTGTTCTTTTGGCTGAATGAGTATATTTGAATTGGTTGAATAATTAATAATTCTCATTGT
  AAAAATAATTATATGCCAAAAATATATTTGATGTTAAATCAAATAGATGATTCTGTTTACATTGTTCAT
  ATGAATAATAATCTGTGTTAATTTCATTTTGATAATTGGCCTTTAATATTTGTATCTCTAATTTTATTT
  TCTCTCTGTTACTGTAAAATAATAGCTATAATGTATAACAATTTTCTTCAGAAGAATTCTATGCTATTA
  TTAAAATAAAATATTTACTGTG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001042390.1
• Summary: The product of this gene belongs to the family of classical tyrosine-specific protein tyrosine phosphatases. Many protein tyrosine phosphatases have been shown to regulate fundamental cellular processes and several are mutated in human diseases. Chromosome 10q contains a segmental duplication resulting in multiple copies of the protein tyrosine phosphatase, non-receptor type 20 gene. The two nearly identical copies are designated as PTPN20A and PTPN20B. A third copy is only partially duplicated and contains a pseudogene, designated as PTPN20C. This gene encodes the more centromeric copy, PTPN20A. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
• Locus ID: 653129
• MW: 62.7