CREM (NM_182722) Human 3' UTR Clone

CAT#: SC215328

3' UTR clone of cAMP responsive element modulator (CREM) transcript variant 9 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CREM
• Synonyms: CREM-2; hCREM-2; ICER
• ACCN: NM_182722
• Insert Size: 1595 bp
• Sequence Data:
  Insert Sequence

  >SC215328 3’UTR clone of NM_182722
  The sequence shown below is from the reference sequence of NM_182722. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAAGATCTTTATTGCCATAAAGTAGAGTAACTGTCTTTGACTTGGACCTTGTTTACTCTAATCAAGGCA
  GGAGATGCAGCAGTCCTACTTATTGCCATGTGGACTTGTGGGAAGGACACGTGTGACCCTTAAGAATCC
  AGTTTGGATTAGTGTTTGAAATTGAATTGGGAATGTTGTTCCAGGATGTGGAATGCAGCCGTGATCACA
  CTTACCGAGCTTACTTTGATCTGTTTGTCAATAGCATGCAAAAAATGCTTTGTTTGCCCTTTGCTTCTG
  CTTTTTTTCAGGGAAGCTGCCAAAGAATGTCGACGTCGAAAGAAAGAATATGTAAAATGTCTGGAGAGC
  CGAGTTGCAGTGCTGGAAGTCCAGAACAAGAAGCTTATAGAGGAACTTGAAACCTTGAAAGACATTTGT
  TCTCCCAAAACAGATTACTAGAAATATTTAACTATGAACTGAAGGCAGCATGTATAGTTGCTTTTGAAG
  GAATACAATATATAGCTGGCAAGAATGGTGGCTTCTTTTCTTTGTATCATTCATCTTCTTCTTTAATCA
  CTTAACATTCCTAAAATGCTTCACTGTACGTAGTTAAGTCGTAGCTATAACTTCAAATTTTTTAAAAGA
  GACAAACTGTAAAAAATGTGTGTATTCTTAAAATGCAATATTTGTAAGGCTTGTTCCAATGCCACATAC
  TTGCAGCTCCCATTCTATGTGTCATCAATAGTGTCCTATGCAATAAAATTATTTGCAGGTCTTTAAATC
  ATTTTAGGAAAGGATGATCAAAAATAATGCATCCAGCAGTACAATAAAAGTAAACCACAAAAAAATACC
  TCAGGAAAGAATAGAAAGAAAGTCTATCTAATGACATGCCTATATGAGAAGAATAGCCTAGATATGAAT
  ATATGGCATTTGCAGATTTTTATATTAGTTGCTTTGTTAAAAAAAAAAAGATTGTATTGCTGTCCTTGA
  ATGCCATAGTCAAAGAGAGTTTTTAATAGAACCATGTTGGTTGCACTTTGTAGTGTTTGGTGCTCATTT
  AAATATCTGAACATTTACTACAGTTTTTAACTCTACTGTGTAATATAAAAGATCTTGCAGAAGTTCTTA
  GTGTTGGTTTAATATTACCTAATGAAAGTGATGACATATTTTTTATATCTGGAATGAGCCTGTTGGGAT
  CGCATTGCATACCTTCGGGGTACAAGTCAGTTTCTACACTGGGTGCTGATCCTTGCACGCGCCCTTTCT
  ACCATCTCACGGTGGGGATGGCCGCAGGGCTGTGCACCCAGAAGAAGATGGCTGTTAGCGTTTCGGCCT
  TCATAATGGCCTGAGACTTTCTTTCTGTAGGTGGTCTGGAGCTGTCCGGCTGGTGGCCCCCTATTTTGC
  CATTTAGCGAACAACCACAGGAATTTTAAAAACAAAAACATCCCAAGATTTTTTCATTTCAAAATGCTT
  CAAAGTCCACATTAGATCAGATACTCCGCTGTCGGCACATTCAGCTGAGGTTCATTACAATCGAGACTG
  CAATGTGATCTATGTTTCATCTTGTTTTTATAATAAAAAGCTTCAGGGAACAAGCCCAAAGCCCTCACC
  ACAAAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_182722.1
• Summary: This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription. [provided by RefSeq, Jul 2008]
• Locus ID: 1390
• MW: 62.1