SERCA3 (ATP2A3) (NM_174957) Human 3' UTR Clone

CAT#: SC215221

3' UTR clone of ATPase Ca++ transporting ubiquitous (ATP2A3) transcript variant 6 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ATP2A3
• Synonyms: SERCA3
• ACCN: NM_174957
• Insert Size: 1579 bp
• Sequence Data:
  Insert Sequence

  >SC215221 3’UTR clone of NM_174957
  The sequence shown below is from the reference sequence of NM_174957. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AACCACATGCACGAAATGAGCCAGAAGTGAGCGCTGGGAACAGAGTGGAGTCTCCGGTGTGTACCTCAG
  ACTGATGGTGCCCATGTGTTCGCCTCCGCCCCCCACCCTTGCCACCACACTCGCCCACTTGCCCACCGG
  GTCCCGCCGGATAAATGACAGGCCCGAGGTCAGAATGGCCATCCCCGGGCCCCGTCCTGGGGTCTCTGT
  CCCCACTTCCTTCTGGCCTGGGAGGTCTGTAATTCCTGTCTCCTGGACTCTCCTGGGAAGTTCCCTGCT
  CTGCAGCTCTGGCCCAGGAGCTGCAGGCTGGGAGGGGGCAGCCAAGAAGCCGGAGCTGGCAGCATACCC
  AGAGATCCGGGGCCCCCCCACCCCCAAATCACGAGTGCAGCTGGAGCTTGCTCCCCCTTGTTCGGAAGC
  TGGACGTTCACTTGGTGACTGGTGCCTCTGCACTGACGGAGGACTCTGGGGGTCCTTCTTACCGGCTCT
  GACCTCTCTCTTCGTGCCTGGTCTGGGACTGGGTCAGCCCTGGGGGATCAGAAGGGGCCATCTGGGCCC
  AGCTGTGTACAGCGAGGGTGGGCAGCCCCCTCCACTCCACTCTGCTTCCACAAAGTCGGCTCCCGAGAG
  CTCGAGGCTGCTTCTGTTTATATGTGCAGGGCCCGGGCGGGTGAAGGGTCAGAGAGACGGACACAAGGA
  GCCGGCAGGAGGGCGGAGCGAGGATGTCCTTTCCCGGGAGACAAGTCGGGAAAGCCTGGCTGGACTGCC
  TCAGCCCCGCGCGCCTCCTGGACTCAGGGTTCCCCGTCCTGAGCTCGGGAGATGTTCAGAGTCACACTG
  CCGCCCGGTCTGCCACGCAGAGGTCCAACTTGCCACCCGCGTCCCTGGTACCTGAGACCACCGACATCC
  TCAGGTTCCTGACCGTGGCGCCCTTCTACCCAGCCCAGTGTGCGGCCGCCGCGCTGTCTGCACAGCTGG
  GGGCCTCTGAGCCTGGTGGGCTTCCTGGACTCTTGGCCTCACTCCTTGCCCCCTCCCCACGACACCCAT
  GAGCCGAAAGGATGTCACTAAGGATGGCTGATTCCCCAAGGGCACCCGCTCTCCCTCCCTCCCTGCTGG
  AGGAACACGTCATATCAGATGAGAGGAAGATGGCCTCTGATGGACAGAATTTTTCTCTTAACTCAGCTT
  TTGCTACTTTGGCAAAAACTAGCGAGGGGTAGCAGAAACCTGCACCAAGGATTGTCCCTATGTCTTGGC
  CACTCCTAGAGCGTGTGCAGACTGATGATTTTATATGTAAATCAAGACTCACATCCCTTTCCTAGTCCC
  CCACATCCAAAGCCCCTCAGCCTGCCTTGCAGACCAATGGGCTCCATGTTCTGTAGCCCCCTCCCCTAC
  GCCTCACCCCTCCTCCCTCTCACAGGTTCTGGGCGGCCAGTGAGAGAAACGCAGTGGGGGAGGCAGGGA
  GTCTGGTGCCTGCAGAGATTCTCTGCTTCTTTCCTGGGGGGAGGTGGGGAGGTCTTAGCAGGAGCGGGC
  CCTGTACCCACCTGCTGACCTGCTGTTTGGTAGAGAAATAAAGGTTGTGTGACTGGGGGAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_174957.3
• Summary: This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
• Locus ID: 489
• MW: 57.2