HMGN2 (NM_005517) Human 3' UTR Clone

CAT#: SC215021

3' UTR clone of high-mobility group nucleosomal binding domain 2 (HMGN2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HMGN2
• Synonyms: HMG17
• ACCN: NM_005517
• Insert Size: 1488 bp
• Sequence Data:
  Insert Sequence

  >SC215021 3' UTR clone of NM_005517
  The sequence shown below is from the reference sequence of NM_005517. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGCTGGAGATGCCAAGTGAAGTGTGTGCATTTTTGATAACTGTGTACTTCTGGTGACTGTACAGTTTGAA
  ATACTATTTTTTATCAAGTTTTATAAAAATGCAGAATTTTGTTTTACTTTTTTTTTTTTTTTAAAAGCTA
  TGTTGTTAGCACACAGAACACTTCATTGTTGTTTTTGGGGGAAGGGGCATATGTCACTAATAGAATGTCT
  CCAAAGCTGGATTGATGTGGAGAAAACACCTTTCCCTTCTAGTTTTGAGAGACTTCCTCTTGGCTCCCAG
  GAGGAGGGATTCCCTGACTTTGACACACATGGCCACCTTGGCACAAAAGCCTTGTGGTATAGAAAAACAA
  ATTTGTTTTTATGTCCTCTTCTCCCTTTCCATCGTTCAGCATAGACTTAACTCCCTTAAGCCCAGACATC
  TGTTGAGACCTGACCCCTAGTCATTGGTTACCAGTGTGTCAGGCAATCTGGACTTTCCAGTGATGCCACT
  GAGATGGCACCTGTCAAAAGAGCAGTGGTTCCATTTCTAGATTGTGGATCTTCAGATAAATTCTGCCATT
  TTCATTTCACTTCCTGAAAGTCAGGGTCGGCTTGTGAAAAGTTGTTAAACAACATGCTAAATGTGAAATG
  TCAACCCTCACTCTAAACTTTCCCTGTTCAGAGCATCAGATGAAGACTTCATTGGGTTTTATAGTGGCTT
  TCTGATTTTTGGTAGTCCATTGAAGAAGGGAGTTTGAAAGTTGTTGTATACTGTTAACGATTGTCTGCCC
  ATGTCCTGCCTGAAATACCATGATTGTTTATGGAAAGTATCTTTAATAAAGCTGGATACAGTTTGGCTTG
  GAATGCTGCCTCTGATCTTTTCCCACAAGTGGGGTAACCTGGTTTATCCAAGTCTCTTGGAACAGGGTAC
  GTTCTGCTTTGAGGTACTCCATGTACAGTCTAATGATGATCTTTCACTGATTTATAGTCAGCTTCCAAAA
  CACACAGTGCTAGGTGCAGTTAGGAAGGATTCCAGGAGTAGACTTAGCTGATGGTGAAAGGCTGGGAGTA
  TGGAGTGATTTCTGTACTTGGTTATGCAGCATTCCCTGCAGTGTTTGTTTTTTGCATGACTCCGAATACA
  TGAAGTGTATTAAATGCAGATTTTTGGTAGATCTGCGGTTTTGGCTGGGTAGCTGACAGGAGCCTCTCTA
  CTTCCATCTGCATTTGCAACTTCTATAAAATTAGCAAATTAATAGACTCATGTCAGAGAGTGTCTCTGTG
  AATGTGTACACAGTTGTCCATTTGTAAGGTAGATGACACTTTGCTCCTAAGTAGACCTGCAAACAAAGAC
  AATGGGGTCTTTCCTGATTCCTTTAGTTTGGAGATGCAGAGAACCCCACGTTTCTCTAAGTGAAATGGCA
  AGAAGTTATTTGTTAGAATATGGAACTGGTAACAAATTCATGTTTGCACCTTGAATTTGCTTTCACAGAC
  CTGGCTGTATTGTTGGAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005517.3
• Summary: The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi. [provided by RefSeq, Oct 2014]
• Locus ID: 3151