NEK6 (NM_001166169) Human 3' UTR Clone

CAT#: SC214967

3' UTR clone of NIMA (never in mitosis gene a)-related kinase 6 (NEK6) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NEK6
• Synonyms: SID6-1512
• ACCN: NM_001166169
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC214967 3’UTR clone of NM_001166169
  The sequence shown below is from the reference sequence of NM_001166169. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CAGATGCACATCTGGATGTCCAGCACCTGAGCGTGGATGCACCGTGCCTTATCAAAGCCAGCACCACTT
  TGCCTTACTTGAGTCGTCTTCTCTTCGAGTGGCCACCTGGTAGCCTAGAACAGCTAAGACCACAGGGTT
  CAGCAGGTTCCCCAAAAGGCTGCCCAGCCTTACAGCAGATGCTGAAGGCAGAGCAGCTGAGGGAGGGGC
  GCTGGCCACATGTCACTGATGGTCAGATTCCAAAGTCCTTTCTTTATACTGTTGTGGACAATCTCAGCT
  GGGTCAATAAGGGCAGGTGGTTCAGCGAGCCACGGCAGCCCCCTGTATCTGGATTGTAATGTGAATCTT
  TAGGGTAATTCCTCCAGTGACCTGTCAAGGCTTATGCTAACAGGAGACTTGCAGGAGACCGTGTGATTT
  GTGTAGTGAGCCTTTGAAAATGGTTAGTACCGGGTTCAGTTTAGTTCTTAGTATCTTTTCAATCAAGCT
  GTGTGCTTAATTTACTCTGTTGTAAAGGGATAAAGTGGAAATCATTTTTTTCCGTGGAGTGGTGATTCT
  GCTAACATTTTTATCTACGTTTTATAACTTGGTGAGTGACGATGAGAGCCCTGCACCTGGCCAGAGTGT
  CACAGGCAAAAGGCATCGGGAAGCAGGAGCATCTTCTTGGCAGCCAGGCTGGGCCATCTTCTCCTGGAC
  ACCTGCTGTGTACCAGGAACTTCGTCACCTCCTTGAATGCTGGCGGTTCATTTCATGATCAGTGTTAAG
  CATTTTCCTCCATGGGAAGGAAGCATGGGATATAGAAAAGCGAAGGGCTGTCCTTTACAAATTCTGGTT
  CTGCAACTTCCTAGCGTGACTTTGGGCTTGGGCAAGTTTCTTAGCCGTTCTGAGCCTTCATTTCCTCAT
  CTGTACAATGAGATTAATAGTACCTATCATCTACCTTCAGGATTGCTGACAGACAGAATTTGAAATAAA
  ATATGCAAGTTAGCTAATACAAAAAGTAGATGATCCAAAAATGGTAGCCACTCACCCTTCACAAACTGA
  AGTCCATGGACCACGGAAGTCGAGAATTAATGTACACCTGTATCATGTGTAGGAAACCAGAAATGTGTT
  CCTTATTTCTTGTTCCCAAACAGGATTAACTGTGAAGACTAATTTATAAATGTGAACCTAAGAAAACTC
  CACCTCTGAAGGAAATCATTTGAATTTTGTTTTTGTACGTAAAGTTAACCTTCCAATTGTCTGAGCTGT
  CGTCACTGACTTCATGACAGTCTGGCCCTCCAGACAAGAGCAGCGCTGGCATCGGGCAGGTGATTCCTG
  ACACCTGCTGCCTGCAGGCATTCACTGACCAGGCCTTTCCTGGAGGAAACACCCAGGGCCGGGCGGCTG
  CTGTTTCCACACGTGGACTCGGATCTGCTGTGACACCGTCAGCCCGACAGTCTCTCCATATGCAGCCTT
  TCCTCTGTACTTTTCTCCATGGTTGAAATAAAACAGGGTGACTGGGAGTTACTTAGAATTCATGAAGAT
  TTTAAAATGGCTTTGGAGATTTTGCTTTTAAACCAGTAGATTCAAAACTTAAACAGCGTCTGCAGCACA
  ATTTCTTGAGGAACCTTGAAAAACACAACTTCCCAGGCCCCATTCAGTAATCCCAGGATTTCTTTAAGC
  TCCCCAAATAATTTTGAAACTCATCATCAGCCGAGTTTCTGCCCTCATGAGGTAATTCCATCGTTCTCC
  CCAGCCTGCCCCTGGCAGCTGTAACACAGGAGCTGGCCTGAGAGCAGATTCACCCTGGAATGTTCTCTC
  CACAGAACAATCAAGTCCCTGTCGCCTGCCTAGTGCTTACCACTGAAGATTTTTCTGATTCCAGACCAA
  CTTTTTGCCAACATTCTGCTTCCAGCTCTCTGAGCCCCTGCCGTGTCTCCCCAACACTGCCAGCCCCAG
  CACGCAATCAACCTACTTTGTGCATGCCACCCGCTTTCCACACTGTGAGAACAATCTGCCCAACTGGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001166169.2
• Summary: The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
• Locus ID: 10783
• MW: 74.4