DISC1 (NM_001012958) Human 3' UTR Clone

CAT#: SC214772

3' UTR clone of disrupted in schizophrenia 1 (DISC1) transcript variant Es for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DISC1
• Synonyms: C1orf136; SCZD9
• ACCN: NM_001012958
• Insert Size: 1503 bp
• Sequence Data:
  Insert Sequence

  >SC214772 3’UTR clone of NM_001012958
  The sequence shown below is from the reference sequence of NM_001012958. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCTCGACATCCTGAACCAAACTCCTATTAATGAAACTTCCCAAATTTAAACAAAGCAAGACAAATAGAT
  ATCCACACAGCGTAGATCATGACTTCTTTAGACATCATGAAAAGAAAAAGAGGACCCACGTGGAAGAAT
  ACGCTCATTTATGATCAGGGTAGCATCATATTCCTCTCCCTTTTAACATTATTTCAGGATAGCAGCTGT
  CTGGATGCTGGCCATGGGCTTGAAAAAAACCAGGTGGGCATTTCTGCCCACTGTACCGTTTCTCCCATG
  CGTTTCCCAGTCCCCTGTCATGGCATGAAAAAACCGCTATTGTTCTGTGTTGGGACAATTAGATTTTTG
  TAGGGGGGAGAAACCACCCAAAAGCAAATGGAAAAATATGCTATGATTAATGTCAGTTACGGGAATTAA
  ACTTCGGAGGCCTTTAGGACAATTATGAAGGAATCATTTTCCCCTTGGTGGCAATATGTACTACAGTTG
  TCCCTTGATATCCATGGGGGATTAATTCCAGGACGCCTGTGGATACAGAAATCCACGTGTACTCAAGTC
  TTGTTTTCAGTGGATATGAAAAGTCGGCCCTCCATATCTGCAGGTTTTACACTTGGCGAATGCTGTATT
  TTTGATTCATGTTGGATTAAGGATCTGGAACCCCTGATACAGACTGTATTTATTGAAAAAAAAGTCTGC
  CTATGAGTAGACTCACATGTTAAAACCCGTGTTGTTCAAGGGTCAACCATAACTGCAGGCAGAACATCT
  GGGCAGGAAGCTCCTGCAGGAAGACCTGTAAACTCAGGTCTGTGGAACGGCGATGATCTTCTTCCCTTG
  CTTCCAGAATTATTGCTGTGAGATCAGGTGCACTGGCTACGACCTTACTCTTTGTGCAGAACTTATTTC
  TGGTCTGGTTTAAGTGAACGTTATTCTTTCTGTGCAGTCCTGTGGTTACATCAAAGGTTAAATTTCATA
  TAGCATGTCTGTTATATCAGACTTCTCTGATGGCCTGTCCAGAGCAAACATTTTAAAATATTGGTCCTA
  TTCTGTCTTATAACAGTCTGCTCTGGTGATTTTTGTCTCATCAATTGGATTATGACACTGTCAGCCACT
  GTTAGTGTTATTCTCAAATTCAGTCTTCCATTCATCTCTCCTTCCCTTCCCTGGCAGACAGACTTCTGC
  AGCCACTGGGACTGTTGGGGTGAGGAAAGGCAAGAACCGATGTTTCATTCTCTCCCTCCCTTCTTCCTC
  CCTCTTTTTTGTTGGTTGTGGGGTGGGCTACATCTTCTTACCCAAACAACCAGGAGCATTTGTTGGCAA
  GTTTATTGTGCCCAGTGCCGATGTCTGGACAATTAATTCCCTAGGTCAGTGGCTTTCACAGTTTGTGAT
  GGTGACTCCAGGAAGGAAACTCACCATGACCCTGTATATACATAGAACAAAAATGCAGCCCACTCTGAT
  ATTGTATTTGTTCTATTCTAGTTCATTAAAAGTGAATGTTGGTCTTCATCCTTT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001012958.2
• Summary: This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins. This gene is disrupted in a t(1;11)(q42.1;q14.3) translocation which segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
• Locus ID: 27185
• MW: 57.4