UBE2D3 (NM_181887) Human 3' UTR Clone

CAT#: SC214674

3' UTR clone of ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog yeast) (UBE2D3) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: UBE2D3
• Synonyms: E2(17)KB3; UBC4/5; UBCH5C
• ACCN: NM_181887
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC214674 3’UTR clone of NM_181887
  The sequence shown below is from the reference sequence of NM_181887. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CGGGAATGGACTCAGAAGTATGCCATGTGATGCTACCTTAAAGTCAGAATAACCTGCATTATAGCTGGA
  ATAAACTTTAAATTACTGTTCCTTTTTTGATTTTCTTATCCGGCTGCTCCCCTATCAGACCTCATCTTT
  TTTAATTTTATTTTTTGTTTACCTCCCTCCATTCATTCACATGCTCATCTGAGAAGACTTAAGTTCTTC
  CAGCTTTGGACAATAACTGCTTTTAGAAACTGTAAAGTAGTTACAAGAGAACAGTTGCCCAAGACTCAG
  AATTTTTAAAAAAAAAAATGGAGCATGTGTATTATGTGGCCAATGTCTTCACTCTAACTTGGTTATGAG
  ACTAAAACCATTCCTCACTGCTCTAACATGCTGAAGAAATCATCTGAGGGGGAGGGAGATGGATGCTCA
  GTTGTCACATCAAAGGATACAGCATTATTCTAGCAGCATCCATTCTTGTTTAAGCCTTCCACTGTTAGA
  GATTTGAGGTTACATGATATGCTTTATGCTCATAACTGATGTGGCTGGAGAATTGGTATTGAATTTATA
  GCATCAGCAGAACAGAAAATGTGATGTATTTTATGCATGTCAATAAAGGAATGACCTGTTCTTGTTCTA
  CAGAGAATGGAAATTGGAAGTCAAACACCCTTTGTATTCCAAAATAGGGTCTCAAACATTTTGTAATTT
  TCATTTAAATTGTTAGGAGGCTTGGAGCTATTAGTTAATCTATCTTCCAATACACTGTTTAATATAGCA
  CTGAATAAATGATGCAAGTTGTCAATGGATGAGTGATCAACTAATAGCTCTGCTAGTAATTGATTTATT
  TTTCTTCAATAAAGTTGCATAAACCAATGAGTTAGCTGCCTGGATTAATCAGTATGGGAAACAATCTTT
  TGTAAATGCAAAGCTGTTTTTTGTATATACTGTTGGGATTTGCTTCATTGTTTGACATCAAATGATGAT
  GTAAAGTTCGAAAGAGTGAATATTTTGCCATGTTCAGTTAAAGTGCACAGTCTGTTACAGGTTGACACA
  TTGCTTGACCTGATTTATGCAGAATTAATAAGCTATTTGGATAGTGTAGCTTTAATGTGCTGCACATGA
  TACTGGCAGCCCTAGAGTTCATAGATGGACTTTTGGGACCCAGCAGTTTTGAAATGTGTTTATGGAGTT
  TAAGAAATTTATTTTCCAGGTGCAGCCCCTGTCTAACTGAAATTTCTCTTCACCTTGTACACTTGACAG
  CTGAAAAAAAACAACATGGGAGTAATAATGGGTCAAAATTTGCAAAATAAAGTACTGTTTTGGTGTGGG
  AGTTGTCATGAGGCTGTGTTGAAGTGACTTATCTATGTGGGATATTGAGTATCCATTGAAATGGATTTG
  TTCAGCCATTTACATTAATGAGCATTTAAATGCAACAGATATCATTTCAGGTGACTTAACATGAATGAA
  TAAAAGTCAATGCTATTGGATTGTTTTTTGTTTGACAAGTGCTATCTGTGCCACTGATTTAACTTCTGT
  AGTAACAAGGGCATTACCATTCTTCACCTTTCCTAATTCTGATCCCATAGTTTTACATTTTTCCTGTTT
  ATTTTGATTTTGTTCACTGCTTTATTTCTTAAAGTTCTAGCACATCTGTGACTCCTCCACTTCCACATT
  TTTGCACTGCTTACACTTACGTGCAATCTTATTCCTTGTCTGCACACACATGTGGAAAGCTAGAAATAA
  ATGTTAAAACTTACTTTTTATAAACATTTTAATATGTAGTTTGGACATGATTTATTGACTTAAGGTTCT
  TCTCTAAACTGGAAGTGAAATGCATGCCTTCTGAAGATGTTCTGGCTTTGTTAATTCTGTAATCATTTC
  ATTGGGGAAAAAACCAGCTACGCAGTTTTTCCAATGAGTGAATTTTTTCATTTTGTGTTTTGCTTAAAA
  CGGCTCCTTCAGGGTAGATGTCATACTGCATAACTTTTTTGGATTCAAATTATGAATGAGAAATTAGT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_181887.3
• Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. [provided by RefSeq, Jan 2017]
• Locus ID: 7323
• MW: 77.5