PAX3 (NM_181459) Human 3' UTR Clone

CAT#: SC213793

3' UTR clone of paired box 3 (PAX3) transcript variant PAX3E for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PAX3
• Synonyms: CDHS; HUP2; WS1; WS3
• ACCN: NM_181459
• Insert Size: 1317 bp
• Sequence Data:
  Insert Sequence

  >SC213793 3’UTR clone of NM_181459
  The sequence shown below is from the reference sequence of NM_181459. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGTGGAGAAGAGGAAGACCTGGAGCAATAAAAGACAAATGCAACATTTTAAGGCAATGGTTTCACATGG
  TTACATATCAAAACATCCCAATTCTTTAGTGCTGATCATCGAGGAGCTAGAACATTCCATTTGCTTGTG
  TGCGTGCGTGCGTGTGTGTGTGTGTGTGTGTATTGAGATTTACCCAGACTGGTTTGGGCAAGCAGACTG
  TTCTAAAATATAATAGAATACCCTAAAGTATAACATGTCGATGTTCGATGCTGATACTTCTACAACTTT
  AAAACTGCTCATTTCATGAAGCAAGAATGGAGGAATCCTTGACTCTTACACACTGGAAATTGAAATAAA
  GATAAATGCAACCATTTTTAGGAAGCTAGACTAGCTGTAAAGGACCTCCTAAATAAATTATTTGTTACT
  GTATCCTCATTTGTAATCTAGTTGTCAATAAATGGCCCGGACATGTCTTGCTAACAGGATATGGGCTAC
  CAGGAAGAAGGACTTGTTTCCCTTTCCTGTGTGGGGAGAGGTGTGTGACGTTTTTCCAGTTCACATTTA
  TTTGGTAGCCCACCTGGGTTGGAGGAAGGGCACGGTGGAGAGAAAGTGACATGCATTCACATATCTGCT
  TATCAGTGTGCAATACTGTGTACCTCATGGATGCTTTGGCAATGCCCAAGGCGATATAAGCAAAATCAG
  AAGTATATTTTTGCAGGTGCTTTTATTTTCACAGCCCATAATTCATAGTGATAGAGTGCAGTGATGTTT
  GCCATTTGCTGGACAACCAACACCCGGGTTGCTCCTTTGATTAGAGACCAGATCAGATAATTATGCTTT
  TAAACTGATTACGTTTACATTCTTCTCTTTTCTATGAAAGGCAGGGCAAGAACAGAAGTGTTCACATGG
  CTCTCATGTACTTTTAAATTATACAATGATATCCAATATTAAAAGATTATAGGAACTGAGGTTTACATA
  CGTTTGTGTGGTGACATTTTAGAATGTCAATAAATTTGCTTTTTGAGATGTTGAGAGGAACAAGAAGGT
  GGAAGGCAGTGTTATTTATTTCTTTCTATTCTTGGAACCACGAATGAGGTAGGCACAAATACATTCCTT
  TTAGAGTTAAGAACTATGACGTAGCGAGTTAGTGAGACTAGACATAGTACCGAATTTAGTTCATGCATT
  GGTCTTAGAGGGTGATGGAACAAGAGAAGTACTATCTTTGTGTTGCAGCCTCAACCAACAACAATGTGT
  TGTAAAAATGTCTTTCATGTAAAAAGTGCAGTAAATATTTACTATTTATAATGAATAAACAGTTATGAA
  AACTTG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_181459.4
• Summary: This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008]
• Locus ID: 5077
• MW: 50.6