TBC1D32 (NM_152730) Human 3' UTR Clone

CAT#: SC213731

3' UTR clone of chromosome 6 open reading frame 170 (C6orf170) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TBC1D32
• Synonyms: BROMI; C6orf170; C6orf171
• ACCN: NM_152730
• Insert Size: 1307 bp
• Sequence Data:
  Insert Sequence

  >SC213731 3’UTR clone of NM_152730
  The sequence shown below is from the reference sequence of NM_152730. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ATGCGGAACATTAGACTGCAGAGCACATAGATCATGAGACACACGGTTTAAATTTAGGTTTTATTTATT
  TTTAAACACAGCAGGGGGGCTTGATGTTTTTCTGTGTCTGTAACAACATTTACTTTGTGAATATACATA
  TTGTAAATACTGAGAAGTATAACGATATATTTAAGTAGGTATGAGCTCAATTTGTGAATTCATTTTTGT
  AAATTTGTTGTTTTGTAAGGTTATTATAGAATCAGATCTAGCTTACTTTTAGTTCTTATTCATGTTTAA
  GAGTTAGTCCTGGCCAGGCGCGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGTCTGAGGTGGGCG
  GATCACGAGGTCAAGAGATCGAGACCATCCTGGCCAAAATGGTGAAACCTCGTCTCTGCTAACAATACT
  GAAATTAGCTGGGTGCAGTGATGCGCCTGTAGTCCCTGCTACTTGGGAGGCTGAGGCAGGAGAATCGCT
  TGAACCCGGGAGGCGGAGGTTGCAGTGAGCCAAGATTGTGCCACTGTACTCCAGCCAGGCCACAGAGTG
  AGACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAGTCCCAACTTACATCTCCTTTATTCAGATGATTTAA
  ATATTGTTTCCAGTGAATTTGGAAAGGAGAAGTAATAGTGTAAATAATATTTTGACTAGCTGCAGAAAG
  CCCATAAGACAAGGAAAAGACAGTATTTCTTCCATTCTTTATGTCTGTACATGTAAAGGAAAATGGATA
  AAACTACAGCTGCTTCTTTTACATGTGGAAGAACAATGATACTATTTACCATGGCAAGTGGTAGGAAAA
  CTGTTGTCCTTGGACATAATTGTTTTTTAGGAGTTGCTTTTGATACCCATATCAATTTATAATTCTTTG
  TTTGAAATGAAGTCTTTACATGGTTCATTGAAGAGATAGATTGGTTATTTCATACTGATAAGCATTCTA
  CTCTTATTTGTTATGCATTTTCCTTAGTGATATATTTTACTTGTACTGAACTTGAAAATATAAAGGAGA
  ATACATTTCTAAATTATTTTAAATGGCTAACACTATGATTTGTCTTATTTAAATAGATGTCTCTGCACC
  GGTAAGATTAATACAATATGTGAATGTCTATTTTTTATATCTTAACTCACAATGAGTATATGAAAGATA
  ATACACGAATATATTACATTATTCATTTTTAGTCATGAGTTTATTTCAATAAGTTTTTCTAATTGTAGA
  TACTGTTTTTTATTCTTTCCTTGTATCTAAATATAAATCAACCATTAAAATCATTCTAACTCTAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_152730.6
• Summary: This gene encodes a TBC-domain containing protein. Studies of a similar protein in mouse and zebrafish suggest that the encoded protein is involved in sonic hedgehog signaling, and that it interacts with and stabilizes cell cycle-related kinase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
• Locus ID: 221322
• MW: 50.7