Pyruvate Kinase (PKLR) (NM_000298) Human 3' UTR Clone

CAT#: SC213725

3' UTR clone of pyruvate kinase liver and RBC (PKLR) nuclear gene encoding mitochondrial protein transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PKLR
• Synonyms: PK1; PKL; PKRL; RPK
• ACCN: NM_000298
• Insert Size: 1309 bp
• Sequence Data:
  Insert Sequence

  >SC213725 3’UTR clone of NM_000298
  The sequence shown below is from the reference sequence of NM_000298. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AACATCATGCGGGTGCTAAGCATATCCTGAGACGCCCCTCCCTCCTCTGGCCCAGCCTACCCTTGTACC
  CCATCCCTTCCTCCCCAGTCTACGTTCTCCAGCCCACACCCCTCCAAAGCCCCACCTTTAAGTCCTCTC
  TTCTCTATTCCTGACCCTCCCTACCTGAGGCCTATCTGAGACTATAACTGTCATCTAGCCCCTTCGAGG
  TTGCCCCTTCCCCATCTCCATTTCACACAGGTCCTGAAAGTCTGTGTCCAATTATGCACTGGCCACCCA
  ACAGCACCAATTGTACATTCCCTGCATCCAATCTGCTCAGCAGGCCCTAAGATGCCTTGAGTCTTTAAT
  CCCAGTTTGGCTGGTTAATTCCATAACCCCAGGCATCCCATCCCTTGGGGTGGGGGAGAGGGGAGACAG
  GGCAATCTTGTCCACAGTCTCCCATTCTCATATGTAGCCCTCATGATAATCTGGGCATCTCGTGCCAGG
  GCAGGCTACCCCTTCATGGTGACTAACAGTTACATGAAAGTCCACGCTTTTGGGAAAACTGGGTGGGAT
  GGATGCTGGGGAGAAGTGAGGGCTGGGCAGCTGATTTTGTCACTGTCTTCACAACCTCCGTGCTGGGCT
  TGTAGACCACTGTCCTGGCTGCTCTCATGCCTGCCTGATACCCTGCTTGGTCAAATCCCCGGCTGCTTC
  CTTCTGCACCCAGAAATTCCTTCCCACTCATGTTGTTCCCACACACAAACCAAGAGCCAAAAATGAGTG
  TGTCTATTTTATATTTAAACCCAGCTGTTTGGAAGCAATTATAAAACTTCTCCCACACACCAAGAACCC
  CAGAACTCCCCACCCAGAAGGAAAGGAGACTTAGGGTCCTGGTCCCACAGTCTTTAATGCTGTAGAGTA
  GGAGAGGGGACAGGCAGAGTGAGGGTGGTAAAGGACTACTCTTGTCCCTGAGAAGGCAGAGGTGCTGGC
  TGGCCTGCCCTTCCCCAGGCTTGGATACCTTGGGCCTTTCCCTTATTCCTGCACCAACAGCAAACTCAG
  AAGGAAAAAACAAAACAAAACTCTAAAGGTAAACGCGGTCCTTCTCCCTCTCTCATGTGGCTCCTCCTG
  CCCCTACCTGGGAGAAGGTTCAAGTATTGCGCTGATGGGTTTGGGCAAGGACCACAGAGGCTGGCATGG
  AGAGGCCCTGCCTCCTCCTACTCCTCCTGTCTCCTTACCTGAAAAGGAGGGGAGGGATGGATAAAGGAA
  TGGGTAAACAAAATTAATTGGTAACACATAAAGTTAGTAAATGAATAAATACAATTAAATAAATGAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000298.6
• Summary: The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 5313
• MW: 48.2