ZNF384 (NM_133476) Human 3' UTR Clone

CAT#: SC213418

3' UTR clone of zinc finger protein 384 (ZNF384) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ZNF384
• Synonyms: CAGH1; CAGH1A; CIZ; ERDA2; NMP4; NP; TNRC1
• ACCN: NM_133476
• Insert Size: 1267 bp
• Sequence Data:
  Insert Sequence

  >SC213418 3’UTR clone of NM_133476
  The sequence shown below is from the reference sequence of NM_133476. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ATCCAGGTGGAGCACCTGGCCAGCTCTTAGAGATCCGTGCTGCCACCCACTGGGAAGAGGAAGAAGTAG
  TCCTGGTGTCTTCTTTCTCCAACTCTTGGTGGGAAAAGTCCTTTTCTTCCTTGACAGGCCTTGGCTCCA
  TCTCCTTGGGCCTCTGTCACGGCTTTCCTTCACAGGATACCATCCTTTTTCTGAACTCTTCTTCAAAAG
  GAACATCAGCCCTCCTGATTGCAAAGGAATACTGAGCTGATGGTGTCATCCAGCAGCCTCCCCTCCCAA
  GCAAAGCTTCTAAAACTGGGGGTCGGTGCTCAAGGGAAGGATTTGCTATGACCTCATAGAACCTTGTCC
  AGTGTGGCCACTTACCCTATCCTTACCCTCCTTATCCTCAAAGTTTGGGCTGATGTAAGACTAGAGGCT
  GGCCCTCCCAGATAACAGAGAAAAGGGAGCCCCAAATGCAACCAGCCTCTTGTTCTATTCTTGCCTGCA
  AAAGAACAGAGGTTTCTCAAATGCCTCAGTCCCTGAGAGCCATTTCTTCCCCTACATCGTCTCACTTTG
  CTTCCTATTGACTGCTGGTAGAAGGAGATTTGGGGTAGGGGCTAGACCTCCTTTTATTTGAAGGGGGCA
  AGGGCTGAGATGTGGTCCCCAAGGGGCCAGAAATTCCCAAGTTGGTCACAGGTGGCTTAGAAGTGTGTG
  TTATGGTTTTACGGATTTCCTTGAAGCCTCTCTCCTTCTCTGCCTACAAAGACCCTATACTCTCAGTCT
  CCCCAACCCACCCCCAAGGAGCTGTGGGAGGCTTTGTGTTATCTGTGAAACTCCAAAACAGGGGTGTTG
  CGGAGAAGGGAGAGTTCAAGGCAAACGCAAGGACTGGACTTAGCTCCCTAGGTGCCACAGTCAGATGCC
  GGACACGGATTTATATATAAATATATATATATAAATATATTATACTCACTCATCACGGCCATCTTTGTT
  GTAACCATTTCTGTGTTTATAAATGCATTATCTCTGAGAATTTTCATATTTGATGTTTTGTTTATTTTT
  GTCCTTTTTTTCCCTCTCTCCACCCCTGTCCTCTAGCCACAGCATTTTTCTTTTTGTCTTTTTTTTTTT
  TTTTTAAATCATGGCAGATTTCAGAGGAAAGGAAATTAAAAAAAAAATCAGGAAACCAGTTGTTATAAA
  GTAATTTAAAAATGAAGAAAAAAAGAAAAAAACTTATGTACAAACCAAGGGGTGTTTTTAGAACATTGT
  ATAGAAATAAATTCATGTAAAAGGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_133476.5
• Summary: This gene encodes a C2H2-type zinc finger protein, which may function as a transcription factor. This gene also contains long CAG trinucleotide repeats that encode consecutive glutamine residues. The protein appears to bind and regulate the promoters of the extracellular matrix genes MMP1, MMP3, MMP7 and COL1A1. Studies in mouse suggest that nuclear matrix transcription factors (NP/NMP4) may be part of a general mechanical pathway that couples cell construction and function during extracellular matrix remodeling. Alternative splicing results in multiple transcript variants. Recurrent rearrangements of this gene with the Ewing's sarcoma gene, EWSR1 on chromosome 22, or with the TAF15 gene on chromosome 17, or with the TCF3 (E2A) gene on chromosome 19, have been observed in acute leukemia. A related pseudogene has been identified on chromosome 7. [provided by RefSeq, Apr 2011]
• Locus ID: 171017
• MW: 48.1