PLAGL1 (NM_002656) Human 3' UTR Clone

CAT#: SC212652

3' UTR clone of pleiomorphic adenoma gene-like 1 (PLAGL1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PLAGL1
• Synonyms: LOT1; ZAC; ZAC1
• ACCN: NM_002656
• Insert Size: 1159 bp
• Sequence Data:
  Insert Sequence

  >SC212652 3’UTR clone of NM_002656
  The sequence shown below is from the reference sequence of NM_002656. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTGCCTCATTTCCATCATGCATTCAGATAATTGATTTTTAAAGTGTATTTTTCGTATTCTGGAAGATGT
  TTTAAGAAGCATTTTAAATGTCAGTTACAATATGAGAAAGATTTGGAAAACGAGACTGGGACTATGGCT
  TATTCAGTGATGACTGGCTTGAGATGATAAGAGAATTCTCGAACTGCATGTATTGTGCCAATCTGTCCT
  GAGTGTTCATGCTTTGTACCAAATTTAATGAACGCGTGTTCTGTAATCAAACTGCAAATATTGTCATAA
  CCAACATCCAAAATGACGGCTGCTATATATAAGTGTTTGTCATATGGAATTTAATCGTAAGCCATGATC
  ATAATGTTAACTAAATAACTTTATGTGGCACTGCCTAGTAAGGGAACTATGGAAAGGTTTGGATTTCTC
  CAAATCTGGGAGAATTTTCAAAATAAGAAAATAACCTTTATATGATATACTATGACTAGGCTGTGTATT
  TCTTTTCAGGGATTTTTCTACCTTCAGGGTTGGATGTAGTTTAGTTACTATTACCATAGCCAACCTGTA
  GTTTTACATATACATTTTCTTGTGGAGCAATAGAGTTCTCCATTTTACAGAAGCATTTTAAATGTAGTT
  TGAATATTTTCCACAAGATGCTGCAATGTGAGTTATCACTTCATTTATCTTAAAGAAAGACTAAACTGG
  TTGTCAGTTACATCTGACAGAAAAAAAAAAAAAATCACTGTGTAACCAGGTTAAGTGGTAAAATAATCC
  AGGCGTCAGTCAAAGGCATTTTGCTGACTTTAATATTGATTATATTTTTAACAGGAATTTAAGAAAATA
  TTACTGGAATTAAAAATATATATATATTAAACAAGAATTTTCTTTGCTCTGTCTAGCTTAAACTACTAC
  TCAAGCTGCTTAAGTTCTTAAGTATTGTTTGTAATCACCAATAAATAAGTGCATTTGTAATTCATCAGT
  CATTATTAGCTTTTATTAAAAGAAGATTACGTTTTACAATGTAACTATAATCTCTTGAATTTGGTATCT
  TATTAATGAGTTTTAAAGATGTAAAACCTAACCTTTTTTAAAGCTCCATTGTCTTATGTTTTTAGAGGC
  TTTTCCGTAAACATATATCTTACATATAATAAACTTTTCAAATCTTGCAAAAAAA
  AGCGGACCGACTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCC
  CAACCTGCCATCACGAGATTTCGATTCCACCGCCGC
  
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002656.3
• Summary: This gene encodes a C2H2 zinc finger protein that functions as a suppressor of cell growth. This gene is often deleted or methylated and silenced in cancer cells. In addition, overexpression of this gene during fetal development is thought to be the causal factor for transient neonatal diabetes mellitus (TNDM). Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding two different protein isoforms. The P1 downstream promoter of this gene is imprinted, with preferential expression from the paternal allele in many tissues. [provided by RefSeq, Nov 2015]
• Locus ID: 5325
• MW: 45.6