NEIL2 (NM_001135748) Human 3' UTR Clone

CAT#: SC212231

3' UTR clone of nei like 2 (E. coli) (NEIL2) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NEIL2
• Synonyms: NEH2; NEI2
• ACCN: NM_001135748
• Insert Size: 1102 bp
• Sequence Data:
  Insert Sequence

  >SC212231 3’UTR clone of NM_001135748
  The sequence shown below is from the reference sequence of NM_001135748. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GAGGAGCCAGAGCAGTGCCAGTTCTCCTAAGGAGCTGGTGGTGCTCCTCACGGAACCTTGCCGCTTGGG
  GAACCTGACGTCTAAGTGTCCAGAAAGGAGGATGTGGGCAGGGACGGGGTACAGAGGATAGTGTGGGTC
  AGAGGTGCCAGTAGTATAATATTCGTCTCCCTGGAGTTATGTTGAAGGCAGAGTTTTCATAGGGTTAGA
  TTTTTTTTATCCTTTTCTAGTTCAGTTAATTCATCCTGTTGAATTGCACCATCGTGAAAGATGGGAAAA
  ATCGTGATGATGGGTAAGGGGAAAACTTCCCGGAAGGCAATGGGGCAAGGAAAAAGAAAGCCTATGGGA
  AATGGCTGTGCTCCCAACATAGCTTTGCAGATGATGTGGGTTTTTTTTTTTTTTTTGGTTGTTTGTTTT
  GAGAGAGAGTCTTGCTCTGTCTCCCTGGCTAGGGTGTGGTGGTGTGATCTTGGCTCACGGCAGCCTTGC
  CCTCCCTGGCTCAAGCAGTCTTCATCTCAGCCTCCAGAGTAGCTGGGACTACAGGCATGTGATATGATG
  CTCGGCTGATTTTTGTTTACTTTTTAGAGAGATGGGGTCTTGCCATATTTGCCAGGCTGGTTTGAACTC
  CTACAACTCAAGCATTCCTCCCACCTTGGTCTCCCAAAATGTTGGGACCACGGGTGTGAGCCACCGCGC
  CCAGCTAGCTCCTGTGTTTTGTTTTTGTTTTGTAACTTTGGTTGATGTTAAGGCCCTCCATTTTGGAAA
  GCAGGAAAACAGGATTTTTTTTTTTTTATCTTGTTCCCTGGAGGATCCAGGGATGAGGATAGAGTGGCC
  TGAGAGCAGTGCTTGGATTCAGCCTCCTGCTGGGTCCTTCTGCTGGATACAGGCACCAAGAGGCGGTCG
  TGGAGCAGGGAGCTGCGCCTTCCTGGGGTGCCCGGTGGTGTGTAGAGAAAAGCTGCTTGTTTACTCCTT
  AAGTCAATGTATTGGTGACTGTTGATTTGTTGAACAATTCAGGAATCAAGGGCTGTGGAGAAACTCCCT
  CATGTTGTTGGCAACAGGTGAATGAACCTAGAGCGGTGACATGAAAATAAAGCTCACTGTTACTCGC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001135748.3
• Summary: This gene encodes a member of the Fpg/Nei family of DNA glycosylases. These glycosylases initiate the first step in base excision repair by cleaving oxidatively damaged bases and introducing a DNA strand break via their abasic site lyase activity. This enzyme is primarily associated with DNA repair during transcription and acts prefentially on cytosine-derived lesions, particularly 5-hydroxyuracil and 5-hydroxycytosine. It contains an N-terminal catalytic domain, a hinge region, and a C-terminal DNA-binding domain with helix-two-turn-helix and zinc finger motifs. This enzyme interacts with the X-ray cross complementing factor 1 scaffold protein as part of a multi-protein DNA repair complex. A pseudogene of this gene has been identified. [provided by RefSeq, Mar 2017]
• Locus ID: 252969
• MW: 41.3