RASGRP 4 (RASGRP4) (NM_170604) Human 3' UTR Clone

CAT#: SC211702

3' UTR clone of RAS guanyl releasing protein 4 (RASGRP4) transcript variant a for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RASGRP4
• ACCN: NM_170604
• Insert Size: 1012 bp
• Sequence Data:
  Insert Sequence

  >SC211702 3’UTR clone of NM_170604
  The sequence shown below is from the reference sequence of NM_170604. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TCAACTGCATCCTCCAAGCTGGATTCCTAGACATCTCTTGGTCTCCTCTCTTCCTCACTCCCTTCCCCC
  AGTCAGTCCTGAGTCCTGCCGTCAGACTCTGGCAGGGCTCCCAGAGGTAGGCCTCCAGAGGCATCCGCC
  TTCCCATCCACACTGCCTTTAGTGGTACGTCCGTCATCTTTTTCCCTGGAAGTGACTTTCCTCTTTTGC
  ATCCTGGTGGATGCTAATCCTGCTCCCTTTCCCTGCACTTAGTATCTGTGCGGCTGTGTGTTGGATACA
  TTTAGACTCAGGCCTCATTCTACCAACTCCCTACATCTCCATTTCTTTTTTTTTTTGAGACAGAGTCTT
  GCTCTGTCACCCAGGCTGGAGTGCAATGATGCAATCTCAGCTCACTGCAACTTCCGCCTCCTGGGTTCA
  AGTGATTCTTCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCACGCGCCACCATGCCCGGCTAATT
  TTTGTATTTTTAGTAGAGACGGGGTTTCCCCATGTTGGCCAAGATGGTCTCAAACTCCTGACCTCATGA
  TCCACCAGCCTCAGCCTCCCAAAGTGCTGGGAATACAGGCGTGAGCCACCACGCCCAGCTCCTGTTTTT
  CTGCATAGAATGTGCTCCCTCTGACAGCCTATACTTTTTCCCTCTTTATCTCGTTGAACGTCTGTCTTT
  CCTCAACAGGAGCATCAACTCCAGGAGAACAGGGATTTCTATCAATGTCATTCACTGCTGCATCCCCAG
  TGCCTAGAACAGGGCTGGCAGGTGGTAGGCGCCTGACAGATGCTTGTCTGATGCCTGAATGTCTCCTTA
  CCCATGCCCACGGCACAGGATAGATGTGCTATAGGGCAAAGAACTTTGAGGGTCGAGCAGCAGGGGCCC
  ATTCAGTCCCAGGGAGCAGAGACCCCCCCAACCCCTTCACAAACCCCAACACCCCTGACTTGGCCCCCA
  CAGAGAGAGGTCCTACAGCTGTCATAAATTAAATTTATTCTCTGGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_170604.3
• Summary: The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]
• Locus ID: 115727
• MW: 37.3