Collagen XI alpha 2 (COL11A2) (NM_080679) Human 3' UTR Clone

SKU SC211579

3' UTR clone of collagen type XI alpha 2 (COL11A2) transcript variant 3 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: Collagen XI alpha 2
• Synonyms: DFNA13; DFNB53; FBCG2; HKE5; OSMEDA; OSMEDB; PARP; STL3
• ACCN: NM_080679
• Insert Size: 1014 bp
• Sequence Data:
  Insert Sequence

  >SC211579 3’UTR clone of NM_080679 ; The sequence shown below is from the reference sequence of NM_080679. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CTGCTGGGGCCTGTCTGCTTCATGGGATAGGACCGTCTCTGTCTGATCCTGTCCATTCGGAACCAGGCC; CACCTGGAATCCCACAACATCAGCTCTGTGCCACCTCCCAAGAGGGCTCCTCACTATCTAGGGAGCCCT; GGGCCAGGGCGTGGAGAGCCCTCAGTCGGGGCAGGCCAGGGGAGGGGTGAAGTGGTTGCCTGGACACCC; CACGGGAGGAGTGGCATCTGGGGCTCTTGGCCCTCCCACCTGGAGCCTGTTACCCGTTAGAGAGCTGAG; ACCCTTATTTAAAACTCACCTCCCAATCACCCCAAACAAATGGAAGAGAAGAGAAAGGACATGGCGTAT; TTTGTATTTAAAAGTAATTGTATTAATTATTTAAAGTGTGGAAAGCAAAATAACAAAAAAGAGAAACGC; CAACAAAAATCAGCAGATGTTGAAGACAGGGGTCTCGGGGGTGGGCTCCGGCACCCACATCCTGGAGTC; AGGACTTTCCTCAGTGACTGTGTGTAGGGGGGTTTCAGGGCTGAACCCCACCTCCCTCCCACCTTCCTC; CCACCTCACCTGTCGCACCCACTGTGAAAGTTGGAATATGTGGTCTCCCTGGCCTCAGGGCTCTGACTC; TGCCAGGGTGGGGCTCTCTAACCCACAGGTGTTGGCTGCCTGGCCCATGTGCCCACTGTCTCTTCCACT; TGGTCTGGGTTTGGCAGGCACTGCCTGCTACTTGAGGGCCAGGATGCTCCCCCAGGGAAGAAACGGAAT; AGTGTGGGGTGTGTGCAGGGCTGCATCCCGCAGATGGCTGGAATATTAAAATTCTTCTATATTGGCTGG; TAAATTGCCATGGCCCTGAGCCACTGAGTATGTTCATTGCCACCCCTGTCCCTCCCCTGGGCACCCCTC; ACTTTCCCTGATCCTGCAATTAAAGGGTTAATGTGTGGCATATGGAAGGGACTCCCAGGACCCTGTGCC; CAGCTTCCATGCTGACTGATGGTTAAATAATGTGATTGTCTCCTCCCA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_080679.3
• Locus ID: 1302
• Summary: This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. It is located on chromosome 6 very close to but separate from the gene for retinoid X receptor beta. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Proteolytic processing of this type XI chain produces PARP, a proline/arginine-rich protein that is an amino terminal domain. Mutations in this gene are associated with type III Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED syndrome), Weissenbacher-Zweymuller syndrome, autosomal dominant non-syndromic sensorineural type 13 deafness (DFNA13), and autosomal recessive non-syndromic sensorineural type 53 deafness (DFNB53). Alternative splicing results in multiple transcript variants. A related pseudogene is located nearby on chromosome 6. [provided by RefSeq, Jul 2009]