ZBTB20 (NM_015642) Human 3' UTR Clone

CAT#: SC211026

3' UTR clone of zinc finger and BTB domain containing 20 (ZBTB20) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ZBTB20
• Synonyms: DPZF; HOF; ODA-8S; PRIMS; ZNF288
• ACCN: NM_015642
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC211026 3’UTR clone of NM_015642
  The sequence shown below is from the reference sequence of NM_015642. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CACATGAGGATGCATGTGTCTGACGGATAAGTAGTATCTTTCTCTCTTTCTTATGAACAAAACAAAACA
  ACAACAAAAAACAAACAAACAAAAAAGCTATGGCACTAGAATTTAAGAAATGTTTTGGTTTCATTTTTA
  CTTTCTGTTTTTGTTTTTGTTTCGTTTCATTTTGTACTACATGAAGAACTGTTTTTTGCCTGCTGGTAC
  ATTACATTTCCGGAGGCTTGGGTGAATAATAGTTTTCCCAGTCTCCCTCGGATGGTGGCCTTAAGGCCT
  GGTAGTGCTTCAAGAGGTCCACTGGTTGGATCTCTAGCTACTGGCCTCTAAATACAACCCTTCTTTACA
  AAAAAATCTTTTAAAAAAAAGTAAAAAAAAAAAAAAAATTTCCACTTGTGAAGAGCACTACAAAAAATA
  TATAACAAAATCTAAAAGGCCTACTGTCTTTAAGTACACCGCTTGCAGTGTTTCAGTGGACATTTTCAC
  AATTCTGGCCGCTTGGACTTCACAGTAACCAGTTAAAACTGTGGAATATCACTTCTGGTTGAAAACCCA
  GAGGAAAGGCCCTGCTGTTTTCCACCTACCACGTTGTCTGATTTCATAAAAGGGCTGTGGGGGTGGGAA
  GGGCAGTGGGTTCGGTGGTGTGGGAAAGAAAGACGAATGGCAGGCTTCTTCCCCAGATTCTGCCCGGGT
  CCACACACCCTGGCCCACCTTCTCCATATCCCCCTCTTGCAGCAGAAGCCAGGAAGACTTGGACAAGCA
  ACAAGCAACAGTGGCTATCGTATTTATTCAGTGTCTTCGCTGAGCCACAGCCTCAGCACAATCAAGAGG
  GACTTTCATGAAAGGCAGGAATGCAGATAAAACAAAGATATCAGAAATTTGCACCTATGTTTCTAGGTA
  CAAGAGAAGGATTATTTCCAACAATCTTTGCAAAAAAAAAAAAAGTGTCAGGATATATTCTTGTGGAAG
  AGAAAAAGAAAAAGAAATGGAGGGTGGGGGGAACAATAAAAAGTTCTTGAGGCTTTTTTAATTCAAAAT
  TTTATAGAGGGGCAAAAGTGACGTTTACCAGATAGAATGCTGATTTTTTTAATATATTTACAACAGTAT
  TTGTGTAAAAAAAAAAACAAAAAAAGTGAAATTGTTAAAAGTAATTTTTTTCATTTTGGTTTTGCATAC
  ACTGCCACCAATTCCTTCTCTTTTATTTTATTTCACACACATATATATATTTTTTGGTAAGTCAAGACT
  GTTAAGGTTAGCGATACTGCTTCCAGATAGAAAGAATAAAAGGCAACTAAAGTTATATTTGAAAGAGAG
  GAAGGATATTTTCTTCATATTTTTTTTAATTTTTCTTAATTTTTATTATTTTAAGTATTGCCTGGGTTG
  ATGAGGGCCTCTGTGGCCAGCCCATCCCTGCTGTAATTTGAATTGCTGCTTTGTATTTTGATATGTAGT
  TCTTTGACTTTAGCAAGCTTAAGTTGCCCCACTGAATAATTTTTTCAACTGATTCATCTAGAATTCTGT
  TCTTTTTCATGGAAGAGACTTTATCTTTCAGAAAGAGATTGCTTGCTTCTCCAAAATTTCATTGAGATC
  TTACAATTCTAGAATTTCTATTGACTTTACTTTTTTTCTTAGTGAAACACTAATAGTCTAGAAGTCTTG
  ATAAATGGGGTCTTTTCTTTTGTGATCAGCCAGGAAGAGGAGTTCACAAAACTCTGCCATAGCTCCTCA
  CCCTTCCCAACTACAAAGAGCATCATTATGAGAAGCCTGTTATAGATCAAGATGCATTGATGCCAAAAT
  TACTTTTTTCCTTCCAAATATCACCTTCTGACTGTTTCCACCATGGTTAAGAGGGCTGAATAAGATGAT
  CATTCTTTAGATGACGAATTAACCCTTGCTTCTTCGAAAGGTTTTAGGGAAATTAACAAAAAAATTCCC
  AGATGCCAACAGCCACCATTCAAAAGACCACCAATCTATTGCATCATACCAGATGCCACTCTCTCTTC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_015642.7
• Summary: This gene, which was initially designated as dendritic cell-derived BTB/POZ zinc finger (DPZF), belongs to a family of transcription factors with an N-terminal BTB/POZ domain and a C-terminal DNA-bindng zinc finger domain. The BTB/POZ domain is a hydrophobic region of approximately 120 aa which mediates association with other BTB/POZ domain-containing proteins. This gene acts as a transcriptional repressor and plays a role in many processes including neurogenesis, glucose homeostasis, and postnatal growth. Mutations in this gene have been associated with Primrose syndrome as well as the 3q13.31 microdeletion syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2017]
• Locus ID: 26137
• MW: 78