DENN (MADD) (NM_001135944) Human 3' UTR Clone

CAT#: SC210924

3' UTR clone of MAP-kinase activating death domain (MADD) transcript variant 10 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MADD
• Synonyms: DEEAH; DENN; IG20; NEDDISH; RAB3GEP; RabGEF
• ACCN: NM_001135944
• Insert Size: 911 bp
• Sequence Data:
  Insert Sequence

  >SC210924 3’UTR clone of NM_001135944
  The sequence shown below is from the reference sequence of NM_001135944. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGAACCCCGCCCCGGCCTGTCTCTAGCTGATGGAGAGGGGCTACGCAGCTGCCCCAGCCCAGGGCACGC
  CCCTGGCCCCTTGCTGTTCCCAAGTGCACGATGCTGCTGTGACTGAGGAGTGGATGATGCTCGTGTGTC
  CTCTGCAAGCCCCCTGCTGTGGCTTGGTTGGTTACCGGTTATGTGTCCCTCTGAGTGTGTCTTGAGCGT
  GTCCACCTTCTCCCTCTCCACTCCCAGAAGACCAAACTGCCTTCCCCTCAGGGCTCAAGAATGTGTACA
  GTCTGTGGGGCCGGTGTGAACCCACTATTTTGTGTCCTTGAGACATTTGTGTTGTGGTTCCTTGTCCTT
  GTCCCTGGCGTTATAACTGTCCACTGCAAGAGTCTGGCTCTCCCTTCTCTGTGACCCGGCATGACTGGG
  CGCCTGGAGCAGTTTCACTCTGTGAGGAGTGAGGGAACCCTGGGGCTCACCCTCTCAGAGGAAGGGCAC
  AGAGAGGAAGGGAAGAATTGGGGGGCAGCCGGAGTGAGTGGCAGCCTCCCTGCTTCCTTCTGCATTCCC
  AAGCCGGCAGCTACTGCCCAGGGCCCGCAGTGTTGGCTGCTGCCTGCCACAGCCTCTGTGACTGCAGTG
  GAGCGGCGAATTCCCTGTGGCCTGCCATGCCTTCGGCATCAGAGGATGGAGTGGTCGAGGCTAGTGGAG
  TCCCAGGGACCGCTGGCTGCTCTGCCTGAGCATCAGGGAGGGGGCAGGAAAGACCAAGCTGGGTTTGCA
  CATCTGTCTGCAGGCTGTCTCTCCAGGCACGGGGTGTCAGGAGGGAGAGACAGCCTGGGTATGGGCAAG
  AAATGACTGTAAATATTTCAGCCCCACATTATTTATAGAAAATGTACAGTTGTGTGAATGTGAAATAAA
  TGTCCTCAACTCCC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001135944.2
• Summary: Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 8567
• MW: 32.8