DNA Polymerase theta (POLQ) (NM_199420) Human 3' UTR Clone

SKU SC210783

3' UTR clone of polymerase (DNA directed) theta (POLQ) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: DNA Polymerase theta
• Synonyms: PRO0327
• ACCN: NM_199420
• Insert Size: 903 bp
• Sequence Data:
  Insert Sequence

  >SC210783 3’UTR clone of NM_199420 ; The sequence shown below is from the reference sequence of NM_199420. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TGGGGAGAGCTAAAGGACTTTGATGTGTAACTGTGCTGTTGATGAAGTCCTCCCAGGGAAGCCTGTGCA; GATGCAGTCACCTGGAAAGAACAGAGATTACCCTTTCACCTACCTCAGCAAAACAAACTTTCAAGTCTT; GATAGACTTAGCCTAGTAATTTTATAGTGAGAGTTTCAAACTATATATCAGTGTCTATAGCATCAAAAA; CTTCTGGGGGCGTGGGGGAAGTAGAATACCAAGTATAATAGTTACATTCACTTTCAAAGAGCATCTATG; AATTTGCCTTTTGTAACTTACTGTGGCTTTAAACATATTCAGAACAGATGCTTGAAATATGCACTTAGC; ACTTTGGTTCCACATCTGTCTGGGTAAACCATGAAGAAAATGAAGCTGCTGCCTCAATCGACCCAGACA; GCAGCCATAGGCAGATAAAGATTTGGTTTCACCCTGGTGGTGGTAGGCATCGTGTGTGACTTTTTTTCC; TCTAATATCAATTTTACAGTACGGAAATAGTATTTTAAAATAGTATTGGCTAATAAATTATGAATTCTA; TAAAGTAGTAAGACTTGGTATGGTTGGAGTGTAGGAATGAATATTCATGAAATGTTTCTTATTGCTTTT; CCTTCCCTAATTCATACAATGAATGTATTTGGAATACTTACATATTATAAAATAAACTATACCTCTTCA; AGAGGTATCCTGTTCTGTAAGATCAGATGTTTTTATTGCAGGTCAATATAATACTGCCAGAGACAGAAA; ATACCCCCTTATCAGTCCCTTAGTGCCTCTTTCTGTTTGTGGCATGGTGAGAAAACCCATGCTGAAAAG; ATTGTACTTTGTGATCCCAATCAGAGGGATGGAGCTAATCTTTTTGCTGTTGAAATAAAATGAATTTAT; GAGAAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_199420.4
• Locus ID: 10721
• Summary: DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963, PubMed:25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25642963, PubMed:25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions (By similarity). The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed:25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed:18503084, PubMed:21050863, PubMed:22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed:14576298, PubMed:18503084, PubMed:19188258, PubMed:24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs (By similarity).[UniProtKB/Swiss-Prot Function]