PTPN12 (NM_001131009) Human 3' UTR Clone

CAT#: SC210049

3' UTR clone of protein tyrosine phosphatase non-receptor type 12 (PTPN12) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PTPN12
• Synonyms: PTP-PEST; PTPG1
• ACCN: NM_001131009
• Insert Size: 819 bp
• Sequence Data:
  Insert Sequence

  >SC210049 3’UTR clone of NM_001131009
  The sequence shown below is from the reference sequence of NM_001131009. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCAAGAGATCCACCTTCAGAATGGACATGATTCAGGGAGCTAGAAGACACTTTAAGTTATACTGGAAAA
  TTCAGGTGCCACTGAAAGCCAGATTTATAGTATTCCATCTTTAATATGTGGGACTAACAGCAGTGTAGA
  TTGTTACCTTAATATTTTTTGCTGGGACCATCTACCTGCCTTATACTACACTTAGGAAAAAGTATTACA
  TATGGTTTATTTTGAAACTTCAAGTATTATTGCCTTAATGTCTCTTAACCCTGTTACACGCTGCTTGTA
  GACATGTTAATATAGTAATACCTTTATGATATATTGAGTTTAAGGACTACTCTTTTTCTGTTTTATCAT
  GTATGCATTATTTTGTATATGTACAGGGCAAGTAGGTATATAATTTGATAAAGTTGCAATTGAAATATT
  ATTAACAGAAGATGTAAGAAATTTCTGCATGGTCTAAATCTTTGTGTACTTTATTTGTAAATTATTTGC
  CCTGGAGTTTTAGAAAATAGTTTCTGAATTTTAAACTTGCTGGATTCATGCAGCCAGCTTTGCAGGTTA
  TCAGAGATCAAAGATTGTAATAATAATTTTGTAAATTGTAAGCAAAAAGTTATTTTTATATTATATACA
  GTCTAATTGTTCATCCTAATTGTTCCTGTTTTCATCTAGTCAGAGATTCAGTAAGTGCCTTGGAACAAT
  ATTGAATTCTCTTAGCTTGTGTGTGTTTCTTTAATATTTGAACTCAAGTGGGATTAGAAGACTATCAAA
  ATACATGTATGTTTCAGGATATTTGACCTGTCATTAAAAAAAACAAACAGTTTTACAGTG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001131009.2
• Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis. This PTP was also shown to interact with, and dephosphorylate, various products related to cytoskeletal structure and cell adhesion, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggests it has a regulatory role in controlling cell shape and mobility. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
• Locus ID: 5782
• MW: 32.3