NCKIPSD (NM_016453) Human 3' UTR Clone

CAT#: SC209643

3' UTR clone of NCK interacting protein with SH3 domain (NCKIPSD) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NCKIPSD
• Synonyms: AF3P21; DIP; DIP1; ORF1; SPIN90; VIP54; WASLBP; WISH
• ACCN: NM_016453
• Insert Size: 730 bp
• Sequence Data:
  Insert Sequence

  >SC209643 3’UTR clone of NM_016453
  The sequence shown below is from the reference sequence of NM_016453. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TTCCTGGTGCTGGGGGAGGCTCCCAGCTAGCACCTTGCTGTCCTCCCTTCCCTGCAGCTCCAGTCAGTG
  TGCAGGGGACTCAGGGGCTTGGCCCTAAGAATGTTTTGCACTGACAGTGGGAGGGGATAAGGTGGCAGA
  AGGAACCTGAGCAGGACCCCCACCTGAAGTAGAACTTAAGAGAGGGGCCAAGTGTAGGATTGGGGACCA
  TGGTCTGGGAGCTGTTCTGGGGCAGGGGGAATATGCAAGCTAAAGCCCCTCTATACCCTGGTAGGCTTC
  CTCTTCAGCATGCCCCCCTCTTCACCCCACACCCATGTGTACACTCAGAGTCCTGCTGCCGCTCCAGGC
  TGGACCAGACCCCCATCCCCACCCCTCCTGGTCTGTATCCTGGGCCTCAGCAAGCCATGCCCCTGGGGT
  GAGGGGAGGCATCTCCCACCAGCTCTATTCTTTGCCTTAGCTTTGCAGTGAGTGCTGCTCATGTCCTCC
  CCTCCACCCGCACATGGGGGTCGCTGCCCTTCACTCCTACCCCCAGAGGCCTTGGCCAAGCTGCTGCTT
  TGAAAGCGGGATCTTGGAGACAGGCCATCCATCCTGGAGCCTCATGGAACAGGATGATGGCACTGAGAA
  AGCCAATGACCGAATCTCTTTTCTCTGTAAAAATGTAGACTGAAAAGCCATGTGTATTTTCCTATGTGC
  TGTAGCTCTCCTTTGGAAATAAATCACAGGCATCTGGAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_016453.4
• Summary: The protein encoded by this gene contains a nuclear localization signal. It plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. It also plays an important role in stress fiber formation This protein is involved in the formation and maintenance of dendritic spines, and modulates synaptic activity in neurons. The gene is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Aug 2019]
• Locus ID: 51517
• MW: 26.4