SDHAF1 (NM_001042631) Human 3' UTR Clone

SKU SC209180

3' UTR clone of succinate dehydrogenase complex assembly factor 1 (SDHAF1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: SDHAF1
• Synonyms: LYRM8; MC2DN2
• ACCN: NM_001042631
• Insert Size: 720 bp
• Sequence Data:
  Insert Sequence

  >SC209180 3’UTR clone of NM_001042631 ; The sequence shown below is from the reference sequence of NM_001042631. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GCACCGGAGACCCGCCCCGACGGACGGTGACAGGCGAAGAGCCGAACTCGCTCGATGGCGTGGTGGAGC; CAGGAGGCTCGCCTGACTGCATGGGGGGACTGGGGAACCCGCCTAAGGTGAGAGGTCTTAAGAGACTAG; CTTGACGAATTGGGGATGTCAGAGACTCCTCCTTGGCGACGCAGGGGGCCTAGAGAGCCCCGTGATGGA; CGGCAAGGGAGGCCCGCCTTTTCCGATGCTTGGAGACAGGTCGGTGCTCCTCCCCCATGAGGGCTTGGG; GCGGCCTGGGACGCTGGCGGGCTGGACAGTGTCAAGCCAAGAGCTACTTGCCCGAAGGTACGGGGAGCC; AGGACGACCCCCGGTGGACAGGGAGAGCCTGAGACGCCCTTCTCTTGACCCCTGAGAACATACCCACTT; CTGGCTCCTCAAGGAGTCTCCCCTCTCCTGTATTTAACTCTGAGAAGTGCAGACTTTTTGCTGAGAACG; TTTTGGGAAGGTGCCCTGATGAGCGGTGAGAAGCCCGGAATCCCCTTCTGGAAAACTTTCCCCCATTAA; TTGTGACAAGCCAGGACCATGAGGAAGGGGTAGGGGTCTATCACCCTGGTTGATCAACTGAAGACCCCC; AAAGGCCCCTACTTGATGGTTTTGAGGGGCAACATTGACTCATTTGCCCCTTCCCTCTCGGAATGTTGG; ACAAAGGGAATAAAATTGGGGATATGTCTA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001042631.3
• Locus ID: 644096
• Summary: The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency).[provided by RefSeq, Mar 2010]