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EFHA1 (MICU2) (NM_152726) Human 3' UTR Clone

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SKU
SC207641
3' UTR clone of EF-hand domain family member A1 (EFHA1) for miRNA target validation
  $683.00
4 Weeks*
Size
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol EFHA1
Synonyms 1110008L20Rik; EFHA1
ACCN NM_152726
Insert Size 588 bp
Sequence Data
Insert Sequence
>SC207641 3’UTR clone of NM_152726
The sequence shown below is from the reference sequence of NM_152726. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
TGGAAACAAGCTGGAAAAGGTCTTTTTTAATAAAAGATATAATAGTATGGCAATTATATTGTTCCAAAT
GTCAAAATTTGTGATTTTTTAGAAGTACTTGCTATTTATCTTCTTAAGTCTTCATTGATATTCTGTGTG
AAATAAGCATGTCTTGTACTTGCTTTCTGATTCATAATTTTATTAAAGAACTTAGTAGAAAGAAAAGTA
AGTATAAAAATAGATATTGGATTCTGTCAGAAGGCCTAGATTTGAAATAATGTTTTGTACTTCGGTAAG
ATGGAAAACTTAGTGATTCACTGATTTCTTAGACACTCTAATATGATATGCTTTCTGGAAGGATAAAAC
AAATACATATGGGAAAAAGTACTTGAGACCAAGGCCAGCATCAATTCCAGACATCTTCATGTTCCTAAT
AGGCTAAATGAAGTTAAAAACTTATTTCAGATTTTTCTCATCTGTACCTTATATCTCATAAATTTATTG
CATATTTTATGTCAGTAGCTTAGCTGTTTATTGTCTTTAAAATAACATGTAAACTTCAATGTTCTATCT
GGAAGCAGAATAAAATATTTACATAGATACAAAAAA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152726.3
Locus ID 221154
Summary Key regulator of mitochondrial calcium uniporter (MCU) required to limit calcium uptake by MCU when cytoplasmic calcium is low (PubMed:24503055, PubMed:24560927, PubMed:26903221). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulate and inhibit MCU activity, depending on the concentration of calcium (PubMed:24560927). MICU2 acts as a gatekeeper of MCU that senses calcium level via its EF-hand domains: prevents channel opening at resting calcium, avoiding energy dissipation and cell-death triggering (PubMed:24560927).UniProtKB/Swiss-Prot Function
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.