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DNA Ligase III (LIG3) (NM_013975) Human 3' UTR Clone

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SKU
SC207544
3' UTR clone of ligase III DNA ATP-dependent (LIG3) nuclear gene encoding mitochondrial protein transcript variant alpha for miRNA target validation
  $683.00
4 Weeks*
Size
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol DNA Ligase III
Synonyms LIG2; LIG3alpha
ACCN NM_013975
Insert Size 2000 bp
Sequence Data
Insert Sequence
>SC207544 3’UTR clone of NM_013975
The sequence shown below is from the reference sequence of NM_013975. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
CGGAAACGGAGACTGGTAGCTCCCTGCTAGGTTTGCTGTCTTCCCTCTCCCTCAGGCCATACTCTCCTT
TACCATACTACTGGACTGGACTCAGGCTGGAGGCAGATAGACACAGTATAGGGGGAATGGGCTTGCTTC
TCCCAAACCCACCAGTTCTCCACTGTCTCTTCTGGACCAGGAATTAGTTGCTGTGGGTGCCACAGCTGA
AGTCAGTTTGTCTTGCTGGTTTAAATAGATCTTTCAGAGCTGGGTGCTGGGTTTGCCATCTTTTTGTTT
TCTTTGAAAAGCAGCTTAGTTACCCTTTTTATAAATAAAATATCTTGCAGTTATCTTTGTCCTTTCCCC
ACCTACACCCCCAATAATTTCCCTAGAGATTAAGGAGTAAAGGCTGGGCTATGGCAGCTCTGTCCACAA
AGCCTTCTCTCCCATCCTTGCCTGTTCCTTTGTACTTCCAGGCTCATTTTAAAGTTGTATTTAAAGGAC
TGCCCTCGGAAATGCTTCTGTTTAGCGGAACTTGTATTCAGCCTGACACGCTTTGCCAGGAACAAACCT
CATGTGAAAGAAAACAAAATGAATTTTTTTACTTTCTTCTCTGTGTTCTCCTATTACATGGTGAGGATC
CCCAGTTTGAAGGGAGGGGACTAGGGTCAGGTAGGAAAATGGGGCCTTTATGAAGAAAGGGGAGGTTAT
TTGGGCCACTCCTCTGGAGGGATAGAGGGCTCCAGGAAGATCTGCATCCCCAAAACCTGGAAACAAGAC
TGTTCTTTAAGAATAAAAATCCACATGGGGCTTGAGGCCAAGAACAGCCCTTGTTGCCACCAACATGGA
GACTTTGTACCATATCCCATTCTTAGTGCTCGAGTGTTCCAACCTGAAGTTGAAGAAGCCACCCTGGCT
GCACATGCCATCAGCCATGACTGTGTATGCTCTGGTGGTGGTGTCTTACTTCCTCATGACTGGAGGAAT
AATTATATGATATTGTTGAACCCCCAAGTATTGGCTGATGAACGTGGGCATCAGAGGCCAGTAGCTTTC
TTGGCCTACAAAGTAAATGGACAGTATATTATGGAAGGATTTGCATCCAGCTTCCTGTTTACAATGGGA
GGTTTAGGTTTCATAATGCTGGACCAGTCGAATGCACCAAATATCCCAAAACTCAATCGATTTTTTTTC
TTCTATTCATTGGATTCGTCTGTGTCCTACTGAGTTTTTTCATGGCTGGAGTATTCATGTGAATGAAAC
TGCCGGGCTATCTGATGGGTTAGAGCGCCTTTAAGAAGAAATCAGTGGATACTGGATTTGCTCCTGTCA
ATGAAGTTCTAAAGGCTGTACCAATCCTCCAATATGAAATGTGGGAAAGAATGAAGAGCAGCAGTAAAA
GAAATACCTAGCGAAAAAAACAGGAAGCATATTGAAGCTCGGACTAGAATTTCTTCTTGGTATCAGAGA
GACAAGTTTATCACAGTATTTTTTTTTTTCCTGCTGACCTATTGATAATACCAACAATGTTGAGTGGCA
TTTTCTTCTTAGTTTTTAATTTCTTAAAGAAAATATACTCCATATCTGCAGGGAAAAAAATAAAAATCC
ACACCTGAGAACAGATCCATACAACCTGCTTACAAAGAACAGCTCCTGTGCTACCTGAGGCTGATTTAT
TTGAAGAACAAAAGGAAGAGAGACAATTTAGTGTAGACAAGTGCTATTCAATAGAACTTTTTGCAGCAG
TGGAAATATCCATAAATCTGCAGTATCCAACCAAACTAGCCACAGACAGCTGCTGAGCAATTGAAATGA
AGCTGGCACAACTAAGGAACTGAAGTTCTTATTTTAATTCAACTTCAAATGGTCACATGTCACTAGTGA
CTACCACACTGGACAGTGCAGGTGTAGACGGAGTTCAGATCAGACTCCTGAAACTTAATGGCTCTAACC
TTAGGCAAGTTACGTTAACATCTAAGCCTCAATCCCTCCAAGTAGAATGGGTGCACCTAAATCATAGG
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013975.4
Locus ID 3980
Summary This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. provided by RefSeq, Jul 2008
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.