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GAD65 (GAD2) (NM_000818) Human 3' UTR Clone

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SKU
SC207536
3' UTR clone of glutamate decarboxylase 2 (pancreatic islets and brain 65kDa) (GAD2) transcript variant 1 for miRNA target validation
  $683.00
4 Weeks*
Size
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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol GAD65
Synonyms GAD65
ACCN NM_000818
Insert Size 589 bp
Sequence Data
Insert Sequence
>SC207536 3’UTR clone of NM_000818
The sequence shown below is from the reference sequence of NM_000818. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
GAAATAGAACGCCTTGGACAAGATTTATAATAACCTTGCTCACCAAGCTGTTCCACTTCTCTAGAGAAC
ATGCCCTCAGCTAAGCCCCCTACTGAGAAACTTCCTTTGAGAATTGTGCGACTTCACAAAATGCAAGGT
GAACACCACTTTGTCTCTGAGAACAGACGTTACCAATTATGGAGTGTCACCAGCTGCCAAAATCGTAGG
TGTTGGCTCTGCTGGTCACTGGAGTAGTTGCTACTCTTCAGAATATGGACAAAGAAGGCACAGGTGTAA
ATATAGTAGCAGGATGAGGAACCTCAAACTGGGTATCATTTTGCACGTGCTCTTCTGTTCTCAAATGCT
AAATGCAAACACTGTGTATTTATTAGTTAGGTGTGCCAAACTACCGTTCCCAAATTGGTGTTTCTGAAT
GACATCAACATTCCCCCAACATTACTCCATTACTAAAGACAGAAAAAAATAAAAACATAAAATATACAA
ACATGTGGCAACCTGTTCTTCCTACCAAATATAAACTTGTGTATGATCCAAGTATTTTATCTGTGTTGT
CTCTCTAAACCCAAATAAATGTGTAAATGTGGACACA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000818.3
Locus ID 2572
Summary This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. provided by RefSeq, Oct 2008
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.