RGR (NM_001012722) Human 3' UTR Clone

CAT#: SC207358

3' UTR clone of retinal G protein coupled receptor (RGR) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RGR
• Synonyms: RP44
• ACCN: NM_001012722
• Insert Size: 1351 bp
• Sequence Data:
  Insert Sequence

  >SC207358 3’UTR clone of NM_001012722
  The sequence shown below is from the reference sequence of NM_001012722. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CAGAAGAGGGAGAAGGACCGAACCAAGTGAGCCTGCCACCCTGGAGTGAGCCCCAGGCCAGGAGGCTGT
  TCCAGGAGTCCTGCCCAGCAGCCTCAGTGGCCAAGCCCAGACACTCACCCACCTTCCCCAGTGGCCCCG
  TGGATCCTGGTCCTAGGCTGGACACAGGATTCAGAAAGACACCAGGCTGCACAGAAAGAGCCAGATGGA
  CCTGAGTGTCGGTCACAGCCCCCTACACTCAAGGCTGAGAGGCCTCAGGAAAGTCATTCCTTTTTAAAA
  ATAATAATAAATGTAAGGGGGTACAGTGCAGTTTTGTTACATGGATAGATTGCCTAGTGGTGAAGTCTG
  GGCTTTTAGTGTAACCATCACCCTAATAATATACGTTGTACCCATTAAGTTATTTCTCATCCCTCACCC
  CCTCCCACCTTGTCACCCTTCTGAGTCTCCAATGTCTATTATTCCACACTCCATGTCCACGTGTACACA
  TTATTTAGCTCCCACTTACAAGTGAGAACATGTGGTATTTGACTTTCTGTTTTTGAGTTATTTCACTTA
  AAATAATGACCTCCAGTTTCATCCATGTTGCTGCAAAAGACATGATCTCGTTCTTTTTTATGGCAGTGT
  AGTATTCCATTCCATATACATATATAAACGTTATATATATGTAACGTTTTCTTTATCCAATCATCTGTT
  GATGGATAGATTCCATATCTTTGCTACTGTAAACAGCTGTGATAAGCATACAAGTGCAGGTTTCTTTTT
  GATATACTGATTTCTTTTCCTTTGGGTAGATACCAGTAGTAGGACTGCTGGATCAAATGGTAGCTCTAT
  TTTTAAGACAGTATGGAGAAATCACCATACTGTTTTCCATAGGGGTTGTACTAATTTACAGTCCCACCA
  ACAGTGTATAGGTGTTCCCTTTTCTCTGCATCCTTACCAATATCTGTAATTTTTTGACTTTTTAATAAT
  AGCCATTCTCACTTGTGTGAGATAAGGTCTCATTGTGGTTTTAATTTCCATTTCTCTGATGACTTTTGA
  TGTTAAGCATTTTTTCATATGCTTCTTAGCCACGTGTATGTATTCTTTTTGAAAAAATGTTTATTCATG
  TCTTTTGCCCACTTTTTAATGGCATTATTTGGATTTTTTTTGTTGAGTTATTTGAGTTTCTTATATATT
  CTGGATAATAGTCTGGATAATATATTCTGGATAATATATAATAGTCTTATATATTCTGGATATATTCTG
  GATACATGGTTTGCAAATATTTTCTCCGGTTCTGCACGTTGTTCATTCTGTTGATGGTTTATTTTGCTG
  TGCAGAAACTTTTTTAGTTTAATTAAGTCCCATTTGTCTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001012722.2
• Summary: This gene encodes a putative retinal G-protein coupled receptor. The gene is a member of the opsin subfamily of the 7 transmembrane, G-protein coupled receptor 1 family. Like other opsins which bind retinaldehyde, it contains a conserved lysine residue in the seventh transmembrane domain. The protein acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. The reverse isomerization occurs with rhodopsin in retinal photoreceptor cells. The protein is exclusively expressed in tissue adjacent to retinal photoreceptor cells, the retinal pigment epithelium and Mueller cells. This gene may be associated with autosomal recessive and autosomal dominant retinitis pigmentosa (arRP and adRP, respectively). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
• Locus ID: 5995
• MW: 52.2