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EIF4A1 (NM_001416) Human 3' UTR Clone

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SKU
SC206995
3' UTR clone of eukaryotic translation initiation factor 4A isoform 1 (EIF4A1) for miRNA target validation
  $683.00
4 Weeks*
Size
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol EIF4A1
Synonyms DDX2A; EIF-4A; eIF-4A-I; EIF4A; eIF4A-I
ACCN NM_001416
Insert Size 550 bp
Sequence Data
Insert Sequence
>SC206995 3’UTR clone of NM_001416
The sequence shown below is from the reference sequence of NM_001416. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
ATGCCCCTCAATGTTGCTGACCTCATCTGAGGGGCTGTCCTGCCACCCAGCCCCAGCCAGGGCTCAATC
TCTGGGGGCTGAGGAGCAGCAGGAGGGGGGAGGGAAGGGAGCCAAGGGATGGACATCTTGTCATTTTTT
TTCTTTGAATAAATGTCACTTTTTGAGGCAAAAGAAGGAACCGTGAACATTTTAGACACCCTTTTCTTT
GGGGTAGGCTCTTGCCCCAGGCGCCGGCTCTTCTCCCAAAAAAAAAAAAAAAACACTAATCCATTTCCC
TAACCTAGTAACCTCCAGATCCCAGAGGCTCTCCTCACCTCAGCTGAGCTCCTTTGAAAGTGATTCAAG
GGACTATGTCACTCAGCCTCATTTGCTGGACCAAATCTGGAGGGAGAACCCCTAAAACCCCTAAGTGAG
GTTGCCCAGGGGGTTGTCCCCAGGTGGGGGGAAGCAGGGGAGAGAAAATGGTAGCCATTTTTACATTGT
TTTGTATAGTATTTATTGATTCAGGAAACAAACACAAAATTCTGAATAAAATGACTTGGAAACTGCC
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001416.4
Locus ID 1973
Summary ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.UniProtKB/Swiss-Prot Function
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.