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D4 (ARHGDIB) (NM_001175) Human 3' UTR Clone

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SKU
SC206824
3' UTR clone of Rho GDP dissociation inhibitor (GDI) beta (ARHGDIB) for miRNA target validation
  $683.00
4 Weeks*
Size
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol D4
Synonyms D4; GDIA2; GDID4; Ly-GDI; LYGDI; RAP1GN1; RhoGDI2
ACCN NM_001175
Insert Size 521 bp
Sequence Data
Insert Sequence
>SC206824 3’UTR clone of NM_001175
The sequence shown below is from the reference sequence of NM_001175. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
CTGTCGATTAAGAAGGAGTGGACAGAATGAATGCATCCACCCCTTTCCCCACCCTTGCCACCTGGAAGA
ATTCTCTCAGGCGTGTTCAGCACCCTGTCCCTCCTCCCTGTCCACAGCTGGGTCCCTCTTCAACACTGC
CACATTTCCTTATTGATGCATCTTTTCCCACCCTGTCACTCAACGTGGTCCCTAGAACAAGAGGCTTAA
AACCGGGCTTTCACCCAACCTGCTCCCTCTGATCCTCCATCAGGGCCAGATCTTCCACGTCTCCATCTC
AGTACACAATCATTTAATATTTCCCTGTCTTACCCCTATTCAAGCAACTAGAGGCCAGAAAATGGGCAA
ATTATCACTAACAGGTCTTTGACTCAGGTTCCAGTAGTTCATTCTAATGCCTAGATTCTTTTGTGGTTG
TTGCTGGCCCAATGAGTCCCTAGTCACATCCCCTGCCAGAGGGAGTTCTTCTTTTGTGAGAGACACTGT
AAACGACACAAGAGAACAAGAATAAAACAATAACTGTG
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001175.7
Locus ID 397
Summary Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 PubMed 8356058).supplied by OMIM, Dec 2010
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.