MRPS7 (NM_015971) Human 3' UTR Clone

SKU
SC206367
3' UTR clone of mitochondrial ribosomal protein S7 (MRPS7) nuclear gene encoding mitochondrial protein for miRNA target validation
  $683.00
4 Weeks*
Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol MRPS7
Synonyms bMRP27a; COXPD34; MRP-S; MRP-S7; RP-S7; RPMS7; S7mt
ACCN NM_015971
Insert Size 483 bp
Sequence Data
Insert Sequence
>SC206367 3’UTR clone of NM_015971
The sequence shown below is from the reference sequence of NM_015971. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
CGTGCCCTGGCCCACTACCGCTGGTGGTAGAGTCTCCAGGAGGAGCCCAGGGCCCTCTGCCGCAAGAAA
CAGTGTGAGCTACTGCCACGCTGAAAACTACCTGTGGGTTAAGGATGTAGTTCCTTTGTAAGGGTGGGC
AGGCCTCGTAAGAAAGATGTAGCAGCATATTCACTATCCGTTAATCCTTCTTTCTTTGAGGCTGGAACT
TGCTCTCTCTGCCCCTATTTCCTTGTAAAGAGGGAGCACATTGACTTGGGAATTTCCTCCAGGAAACTC
AGGGCTGTTTTCTCTTCCCTTAGGTTGGGGCGGACCTTTGGATATATAAAGGAAGCAGTTTTAGTATCA
GAAAAGATTTATTAGAAAATTCTCACGCTGAACTGGTGTAGCATGTGGTGCAGCATTCAGTGAAACTGG
CTGGAGGAAATAGGCTTGTTTCCAGAGTTGTCCTTATACAAAATGTATAAAAAGCAGTTTCTGGTGTGA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015971.4
Locus ID 51081
Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. In the prokaryotic ribosome, the comparable protein is thought to play an essential role in organizing the 3' domain of the 16 S rRNA in the vicinity of the P- and A-sites. Pseudogenes corresponding to this gene are found on chromosomes 8p and 12p. [provided by RefSeq, Jul 2008]
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.