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PPHLN1 (NM_001143788) Human 3' UTR Clone

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SKU
SC206342
3' UTR clone of periphilin 1 (PPHLN1) transcript variant 7 for miRNA target validation
  $683.00
4 Weeks*
Size
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol PPHLN1
Synonyms CR; HSPC206; HSPC232
ACCN NM_001143788
Insert Size 702 bp
Sequence Data
Insert Sequence
>SC206342 3’UTR clone of NM_001143788
The sequence shown below is from the reference sequence of NM_001143788. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
TCCACTCAAGATTTTGGAGAGCCTTTTTAGATTTTTCTGCTCAGGCTAAAAAAAAAAAAAAACAGTTTC
TAAAAATTTTTTTTCCTGGATTGTGTAAATCCTTAATATATGGAATTTTTGTGATGCAGAGAAATACTT
TATGATAGTTGACAACATTTCAGTATTAAATAAACATCTAAAATAGTCTGTTTAAAATGTTTGATTCAA
ATTTTTGTATTTTTTGTAGAGATGGGGTTCACCATGTTGGCCAGGCTAGTCTCTAACTCCTGGCCTCAA
GTGATCTGCCTGCCTCAGCCTCTCAAAGTGTTGAGATTACAGGCGTGAGCCACCGCTCCCTGCCCAACA
CATATACCATCTGAAAATGTTAGAATTCTGAGTTGTGATTTTATTGACTTGTTGCTTGCTTTTTCTTAG
GCTTTGTAACTTGTAATATGTTAAAGTGTACTATCCTAATAAACTGAATACTTTGGTATCTTAGAGAAT
ATTTGCTTTGAGAGTAATTCTCATTCAATGATAAAATAGAGCACTTAAATCTCTGAGAGATACCTCAGA
AAAAAATCCGTTTTTCCAAGTAATGAACTCAGTGTCTTCTATTACAATAATCCTGAAACTCCTGACTCT
CTCACTGATGTATGAGTCTTAAATAATATCATATACAAGACTAATAAATAGAAGATGCAGTTTGAGGTG
GAAGGTGAGGGA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001143788.2
Locus ID 51535
Summary The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.