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DNA Polymerase gamma (POLG) (NM_001126131) Human 3' UTR Clone

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SKU
SC206237
3' UTR clone of polymerase (DNA directed) gamma (POLG) transcript variant 2 for miRNA target validation
  $683.00
4 Weeks*
Size
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol DNA Polymerase gamma
Synonyms MDP1; MIRAS; MTDPS4A; MTDPS4B; PEO; POLG1; POLGA; SANDO; SCAE
ACCN NM_001126131
Insert Size 461 bp
Sequence Data
Insert Sequence
>SC206237 3’UTR clone of NM_001126131
The sequence shown below is from the reference sequence of NM_001126131. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
TTGGAAAAACGAAGCCAGCCTGGACCATAGCACTGCCTGGAGGCTCTGTATTTGCTCCCGTGGAGCTTC
ATCGGGGTGGTGCAGGCTCCCAAACTCAGGCTTTCAGCTGTGCTTTTTGCAAAAGGGCTTGCCTAAGGC
CAGCCATTTTTCAGTAGCAGGACCTGCCAAGAAGATTCCTTCTAACTGAAGGTGCAGTTGAATTCAGTG
GGTTCAGAACCAAGATGCCAACATCGGTGTGGACTACAGGACAAGGGGCATTGTTGCTTGTTGGGTAAA
AATGAAGCAGAAGCCCCAAAGTTCACATTAACTCAGGCATTTCATTTATTTTTTCCTTTTCTTCTTGGC
TGGTTCTTTGTTCTGTCCCCCATGCTCTGATGCAGTGCCCTAGAAGGGGAAAGAATTAATGCTCTAACG
TGATAAACCTGCTCCAAGGCAGTGGAAATAAAAAGAAGGAAAAAAAA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001126131.2
Locus ID 5428
Summary Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.