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Phospholipase C epsilon 1 (PLCE1) (NM_016341) Human 3' UTR Clone

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SKU
SC206072
3' UTR clone of phospholipase C epsilon 1 (PLCE1) transcript variant 1 for miRNA target validation
  $683.00
4 Weeks*
Size
Bulk Requests & Clone Modifications

Frequently Bought Together (2)

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Specifications
Specifications
Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Target Symbol Phospholipase C epsilon 1
Synonyms NPHS3; PLCE; PPLC
ACCN NM_016341
Insert Size 2000 bp
Sequence Data
Insert Sequence
>SC206072 3’UTR clone of NM_016341
The sequence shown below is from the reference sequence of NM_016341. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
TCCAGTGACACAATGGATTACCGACAGTGACTAAGGGCAGCATGTTTAACCCAGGTGAAGATCTTTAAG
CAAGAAGTTAAAGAGTGAACATGGTGGAAAAAATATAATTATTTTCATCAGACTTAAACTGGAAATTGA
TGATTTCTGAACTGAAGCCTTCACACATGTGAGATCCATGCTGAGGAGAAGCAAAATGGCACAGGGCTA
GTTGCCACCAACCAATTTACTGATGAATGAAGCCCAGGGGACTGCCATTTTATAAATGTCAGCAGTTGG
AAAAATCGTCACGAATTGACTTAGAGCAAGGGTCAGCAAGCTTGTCTGTAAAGGGCCAAACAGTAAATA
TTTTAGGGCTGGGGGCCATAAAATATGTTGCAACCACCCAATTCTGCCATTGTAGTGCAAAAGCAGCCA
TAGACAACACATACATGAACGAACGTGGCTGTATTCCAATAAAACTTTATTTATGGACACTGAAATTGG
AATTTCATATATTTTTCACATGTCACAAATACTCTTGTTTTGACTTTTTTTCAACCATTTAAAAACTTA
AAAACTATTCTTAATTCAGGGGCCATACAAAAACAGGTGGCAGGCTAGATTTGGCCCAAGGACCTTAAT
TTGCCAACACAACTAAGAGAAGAGAACTGGGAACAAGGAGCACAAAGATCTGAAAACAGGAAAGGAAGT
TTAGCCATACATTTTATGGCCGAGAATTAGAAGCTTAGAATTAGAAGCTTTGGGGCATTTCAAGTGTAA
ATATGTCCTATAACATAAGAGTCATTAAAATTCAATTTAGTTCCTGTATTGTGTACCACAGTGCACTCC
TCTTTAGGATTTTTTTACATATAGAAATAATATTGGGTTTTTTTTAAGGTTATTGCCTATTCAGTTAAT
AGGGTTGCTTTAAAAAAGGTTATTGCTTAATCATAGATTAGATTACTCATTTTTGGTTTTCAAAATGAA
TTTAGAATGACGTATAATTTAATATCTACTGAATTGCAATGTCTTAACAAGAAACCTAACAATTTTTTA
AAGCAAAAGTAGATTGAGTTGGCGTTTAAATTTATTTTGCAAGGTTTGTACTGACACTATACTTATATA
TTTATTATACATCGCTCTCTTGATATTGCCATGGATTAGAAGCATTAGTTCTCAGTACTTGAAGACAAA
CTTCTAAAAAGAAAATATATGCTCTGAACATCTGAAATGGGCTAGACTTTCAAGTAAAATTGCTTCATT
TCTCATTAACTGAAGAGCTATTGATCCAAGTCATACTTGCCATTTAATGTAAATTATTTTTAAACTTTG
CTGTACAAAACCATTAAGTGTAAAGGATTCTTCATATTCTTAGTATGAAGCAAATTATGAATTTTAAAA
ATGAAATATGTATTCCTCTAAAACCCAAACTTTTATCTTTCCATTGGTATCTTTGAATTTTAAGACACT
TTTCTTCAAATTACAAAACACTAAAACTCACTCCTAAACTTCACTTTGAGAAACTTTTTTAACCAATTA
TAAAAATAAAACCATCCCCATGTGTTTCTTGCATATTCTAAAAACAGAAGTGTTAAAAATACACCACAC
AGGCTGGGCAGAGTGGCTCACGCCTGTAACTCCAGCACTTTGGGAGGCCAAGATGGGCGGATCAGGACA
GAGAATGAGGCCATCCTGGCCAACATGGTGAAACCCAATCTCTACTAAAAGTACAAAAATTAGCTGAGC
GTGGTGGCACGTGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCACTTGAACCTGGGAG
GTGGAGGCTGCAGTGAGCCAAGATCGCACCACCACGCTCCAGCCTGGTGACAGAGCGAGACTCCGTCTC
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACCATACAGCTTTCATGTCATTGAAAGTTCTTA
CTGGGTCAAAGCCTTAAGGCTAGAACTACCATTTTCTTGTCAACAGAATTCAGGCTATTCCAGTTTGA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI |
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016341.4
Locus ID 51196
Summary This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.provided by RefSeq, Sep 2009
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.