PPP2R2B (NM_001127381) Human 3' UTR Clone

CAT#: SC206045

3' UTR clone of protein phosphatase 2 (formerly 2A) regulatory subunit B beta isoform (PPP2R2B) transcript variant 7 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PPP2R2B
• Synonyms: B55BETA; PP2AB55BETA; PP2ABBETA; PP2APR55B; PP2APR55BETA; PR2AB55BETA; PR2ABBETA; PR2APR55BETA; PR52B
• ACCN: NM_001127381
• Insert Size: 477 bp
• Sequence Data:
  Insert Sequence

  >SC206045 3’UTR clone of NM_001127381
  The sequence shown below is from the reference sequence of NM_001127381. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTATATATATTCCAGGACAAGGTTAACTAGGTGGACAAGTTATTACTTAATAATCTCACATACTGAATA
  CTAGTCAAACAAGTTTTTAAATGTTTCTTTGGGTCTTCATTTGATGCATTGACTTTAATTTCCCTATAC
  AGGAAATGATTGGAATAGAATTAAAAGGAGTCCAACATTCCCAGCTCCCCAGTTCTAAGAAACTTTTGT
  CAAACCCAATAGGTTTGGGACACTTCTGTTTAGAATTGAAAGCTGCCAGCTAACAGTAATTCTTCCATA
  GTTGACTTGAACTTCTGATGCTTTTATTGCCCAGTTTTCTCTGGTGGGTCCAGTGTTTTGTTCCTAGGT
  GTCTGCTGCGATAAAATGAGGTTGTCTGTAGTATTTAAGGAGAAAAGAGATAAGTTTTTTTTAATTAAG
  CAATTCCATTTGATTGAAAAAAATCAACAAAAAATAAACACCGTTTACTCTTAGACAAAAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001127381.1
• Summary: The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in this gene cause autosomal dominant spinocerebellar ataxia 12 (SCA12), a disease caused by degeneration of the cerebellum, sometimes involving the brainstem and spinal cord, and in resulting in poor coordination of speech and body movements. Multiple alternatively spliced variants, which encode different isoforms, have been identified for this gene. The 5' UTR of some of these variants includes a CAG trinucleotide repeat sequence (7-28 copies) that can be expanded to 55-78 copies in cases of SCA12. [provided by RefSeq, Jul 2016]
• Locus ID: 5521
• MW: 18.4