HLA DP (HLA-DPA1) (NM_033554) Human 3' UTR Clone

CAT#: SC204584

3' UTR clone of major histocompatibility complex class II DP alpha 1 (HLA-DPA1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HLA-DPA1
• Synonyms: DP(W3); DP(W4); DPA1; HLA-DP1A; HLA-DPA; HLA-DPB1; HLADP; HLASB; PLT1
• ACCN: NM_033554
• Insert Size: 837 bp
• Sequence Data:
  Insert Sequence

  >SC204584 3’UTR clone of NM_033554
  The sequence shown below is from the reference sequence of NM_033554. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CATGACCCCCGGGCCCAGGGGACCCTGTGAAATACTGTAAAGGTGACAAAATATCTGAACAGAAGAGGA
  CTTAGGAGAGATCTGAACTCCAGCTGCCCTACAAACTCCATCTCAGCTTTTCTTCTCACTTCATGTGAA
  AACTACTCCAGTGGCTGACTGAATTGCTGACCCTTCAAGCTCTGTCCTTATCCATTACCTCAAAGCAGT
  CATTCCTTAGTAAAGTTTCCAACAAATAGAAATTAATGACACTTTGGTAGCACTAATATGGAGATTATC
  CTTTCATTGAGCCTTTTATCCTCTGTTCTCCTTTGAAGAACCCCTCACTGTCACCTTCCCGAGAATACC
  CTAAGACCAATAAATACTTCAGTATTTCAGAGCGGGGAGACTCTGAGTCATTCTTACTGGAAGTCTAGG
  ACCAGGTCACATGTGAATACTATTTCTTGAAGGTGTGGTTTCAACCTCTGTTGCCGATGTGGTTACTAA
  AGGTTCTGATCCCACTTGAACGGAAAGGTCTGAGGATATTGATTCAGTCCTGGGTTTTTCCCTAACTAC
  AGGATAGGGTGGGGTAGAGAAAGGATATTTGGGGGAAATTTTACTTGGATGAAGATTTTCTTGGATGTA
  GTTTGAAGACTGCAGTGTTTGAAGTCTCTGAGGGAAGAGATTTGGTCTGTCTGGATCAAGATTTCAGGC
  AGATTAGGATTCCATTCACAGCCCCTGAGCTTCCTTCCCAAGGCTGTATTGTAATTATAGCAATATTTC
  ATGGAGGATTTTTCTACATGATAAACTAAGAGCCAAGAAATAAAATTTTTAAAATGCCCTAAAAAAAAA
  AAAAAAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_033554.3
• Summary: HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq, Jul 2008]
• Locus ID: 3113
• MW: 32.2