HLA-DRB3 (NM_022555) Human 3' UTR Clone

CAT#: SC204177

3' UTR clone of major histocompatibility complex class II DR beta 3 (HLA-DRB3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HLA-DRB3
• Synonyms: DRB3; HLA-DPB1; HLA-DR1B; HLA-DR3B; HLA-DRB3*
• ACCN: NM_022555
• Insert Size: 347 bp
• Sequence Data:
  Insert Sequence

  >SC204177 3’UTR clone of NM_022555
  The sequence shown below is from the reference sequence of NM_022555. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGACTTCAGCCAACAGGATTCCTGAGCTGAAGTGCAGATGACAATTTAAGGAAGAATCTTCTGCCCCAG
  CTTTGCAGGATGAAAAGCTTTCCCGCCTGGCTGTTATTCTTCCACGAGAGAGGGCTTTCTCAGGACCTA
  GTTGCTACTGGTTCAGCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGTTCCTGCCCTTGGCCT
  GAAGTCCCAGCATTGATGGCAGCGCCTCATCTTCAACTTTTGTGCTCCCCTTTGCCTAAACCCTATGGC
  CTCCTGTGCATCTGTACTCACCCTGTACCACAAACACATTACATTATTAAATGTTTCTCAAAGATGGAG
  TT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_022555.3
• Summary: HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. There are multiple pseudogenes of this gene. [provided by RefSeq, Feb 2020]
• Locus ID: 3125
• MW: 12.7