PLCD1 (NM_001130964) Human 3' UTR Clone

CAT#: SC203724

3' UTR clone of phospholipase C delta 1 (PLCD1) transcript variant 1 for miRNA target validation


Reconstitution Protocol

USD 683.00

4 Weeks*

Size
    • 10 ug

Product Images

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol PLCD1
Synonyms NDNC3; PLC-III
ACCN NM_001130964
Insert Size 307 bp
Sequence Data
>SC203724 3’UTR clone of NM_001130964
The sequence shown below is from the reference sequence of NM_001130964. The complete sequence of this clone may contain minor differences, such as SNPs.
Blue=Stop Codon Red=Cloning site

GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
CTCTTTGTGAAGATCTCCCTCCAGGACTAGGCTGGAGGAAGCCAGTGGGGTCCCCCCTGAGTGGGCTGG
GCCCTCTGTCCACATGTGGGGACAGGGCTGGTGTGGCTGCTCCCAGCCTCTTGCTCAGAGCTAGGCCCC
CAAATTGCCTTCAGCCCTAACATAGTGTCTGCTGCTGCCTCCCTGGGGACCAGGAGCTAGCCCAGTCCC
TGGAGCTGTCCTTCATTCCGTTAGGAATAACACTGCAGCCCTCTCCACCCTCCGGCCAGCGAGTGGTCA
AGGATTTTTATAAAAATCACGATAAGATTAA
ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Reference Data
RefSeq NM_001130964.2
Summary This gene encodes a member of the phospholipase C family. Phospholipase C isozymes play critical roles in intracellular signal transduction by catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers diacylglycerol (DAG) and inositol triphosphate (IP3). The encoded protein functions as a tumor suppressor in several types of cancer, and mutations in this gene are a cause of hereditary leukonychia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
Locus ID 5333
MW 11

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.