NCF1 (NM_000265) Human 3' UTR Clone

SKU SC201664

3' UTR clone of neutrophil cytosolic factor 1 (NCF1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: NCF1
• Synonyms: CGD1; NCF1A; NOXO2; p47phox; SH3PXD1A
• ACCN: NM_000265
• Insert Size: 185 bp
• Sequence Data:
  Insert Sequence

  >SC201664 3’UTR clone of NM_000265 ; The sequence shown below is from the reference sequence of NM_000265. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; ACCAAGCGGAAGCTGGCGTCTGCCGTCTGAGGCTGGAGCGCAGTCCCCAGCTAGCGTCTCGGCCCTTGC; CGCCCCGTGCCTGTATATACGTGTTCTATAGAGCCTGGCGTCTGGACGCCGAGGGCAGCCCCGACCCCT; GTCCAGCGCGGCTCCCGCCACCCTCAATAAATGTTGCTTGGAGTGGA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000265.7
• Locus ID: 653361
• Summary: The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]