Choline Acetyltransferase (CHAT) (NM_020986) Human 3' UTR Clone

CAT#: SC200087

3' UTR clone of choline acetyltransferase (CHAT) transcript variant N2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CHAT
• Synonyms: CHOACTASE; CMS1A; CMS1A2; CMS6
• ACCN: NM_020986
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC200087 3’UTR clone of NM_020986
  The sequence shown below is from the reference sequence of NM_020986. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ACGAGGCCCAGCCAGGGACACCAACCTTGACTCCTGCCACTAGGTTTCACCTCCCAAACCCAGCCTCTA
  GAACAGCCAGACCCTGCAGATCCCCACTCCCGTCCCTTACCCCAGCTTTCCACAGCTCCCTGTCCTCAG
  GGTCCAACTCACAGACCATACAGAGACATCACACAGAGCCGGAGTGTTAGGAGGAAAGGGTCCCCTCTT
  CATGCATGGGAATCATCATTTTCAAGGTGGCTTTGGGCCTGCACACTGGGAAATGGGACCTGCCTGGCT
  CAGAGGCAGCCTGGATGCACTGGGGAACCACACTAAGGACTCCTTCTGTGGTCCTTGGAAGCTTAGTGT
  TCATGTCTCCTTCCCTAGCAGGACCTAGTATGTCCAGGTGATGCTTCTGCCCAAGGAAAGGATGAGTCA
  CTCTATTACATGCAACGTACCTAATGAGTTAGGAAGGAAGAGGCTAACTCCAGGTCATTACCTCTTTTC
  TTTTTTGGGGGAGAGGAGGCTGTGTTTTGAGATTCAGAGCATTCTTATCGTGGCATTCCCAGTGTCTCC
  ACTGAGCCGTACAGTCCTACAAGCACCCACTCCTCCCACACACACCACAGAGCCCAAGTCCATTTAAAA
  TATAGTGACTTGGGCCCACAAACACATTTCTGCTTTCGTGCCCAGGGGCAGCCTTCTGTTGAGCTCAGA
  AAATTGTGTCCAGCTATTCTGAAAGGAAAAAAAAATTTATCTGTGACTGCCCTGGAGTTGCTGCCACTC
  TCTGCTTAGCAGGCGGCATCAGGGCCAGTCCAAGATGAGTAAACTGCACAGCCCCAAGCAGATGGTGCC
  TGGTGCCGGTGGGTTTGCAGAGGACCTGGCCCCTTCCCGGGGTCCTGCCATTTGCATTTTTTCTGCATC
  TTTTCCCCTCTCCTCCCCTCCTACATGCTCCAGTAGGTGAAGAGAGATGGTTACTTTGGGTTTTTCCAT
  TATCTGTTTTGTTTTAAGACAGAGATTTTTAAGAAAACCACCCTGACATTAATTTCAGAAATTCTTAGT
  GATTCAAGCAGGACCCTTAGGCAGCTGGGCTCCTTTATTTGGAGCAGGCTATCCAGGGACTCTGACAAA
  AACCCATGTGGTAGAGCCTAGAGCAGGGCTCTCTTCTGGCCTCAGGGACTTAGGGGACAGCTGGCAGGG
  AGCAGGGCTGGGAGGAGGGCACAGCCTCCTCCAGAGTCAGCCCCAGCCCCAGCCCCAGCTCCAGCTCCA
  GCCCCAGCTCCAGCTCCAGCCCCAGCTCCAGCTCCAGCATGGGCAGGACAGGCAGGTCAGCAGAGGCAG
  AGTTGAGATGTCTCCAGAGACTGTGATCATAGGAGAGACACAAGAGGCACTTCCTATTAGAGCACATTT
  TTATGGGAAGTCTAAAGGGCAGAGGGAGGGAGTAAGAAAGCAACCAGAGAAATCTACAGCATAGAGCCC
  GTGTGTTCTGCTCCACATCCCTCCAATCCTGCTGCTTCCTGCTGAGCACTCTGCTCTAGCCCAGCAATC
  CTGTAGGTCTCCCTTCTTTGTGCCACTGTGAAGGCTCTCCACACTTCGACCTGGACTGTCACAGGCTGG
  CAGAGGTGGGGTGGGCATTTGACCTTTGCTCAGCTCTTTGAAAACACAAGCAGCCTCAAGAAGTGAGTT
  CCTTGAACCCCTGGTTTTAGATGGAGGATCTCTATTAGATGTCATGGGAGATTTGGGCACTACCCTTTG
  AACATACAAACTTAAGTCATTGGCCTGAGATTTATGCTCCTTCCCTCCCACGGCCACCTTCCTACTCTG
  CTTAGGAAGTTGCAGCACAAGGGTCCTGGGTTTTCTGGGGAAAGGCAGCCCACCTGCTGTAAGTTGGCC
  CAAAGCTACTTGTCTGTTCTCTCCAAGTGACCACAGTGCAGATCTGAGTTCTCCTCCGGTTCCTTTCCC
  TCCATGGATTTCTGCGCAGACATAGAGCTCCAGCTATGGGGCCAGGCAGCCCCACCAACCTCGGCCAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_020986.4
• Summary: This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
• Locus ID: 1103
• MW: 73.4