SPlink HRP Broad Spectrum DAB Detection Kit

CAT#: D01-6

Biotin-Streptavidin HRP Detection for MOUSE and RABBIT antibody on human tissue

Product Images

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  User supplied image. Mehmet Fatih B. for D01-110 on 04/15/2023: Although this KIT is prepared for human tissues, I have been using it for cat, dog, cow, sheep and buffalo tissues in my laboratory for years. It gives a very clean high signal in both types of tissues. KIT causes background staining and nonspecific staining in rat and mouse tissues (plasmacytes and sera). I usually use this KIT with citrate buffer pH6.0 or proteinase K enzyme digestion antegen retrieval methods. Sometimes I also use it with anti-goat antibodies in double staining. Another important point is that it should be tried to incubate at the temperature and time written in the KIT, especially long strpteavidin-peroxidase (SA-HRP, third reagent) incubation causes background staining. Serum blocking reagent (pre-block) is also very effective. Here is am adding an image of uroplakin III antibody staining with this KIT. I hope it helps... Associate Professor Mehmet Fatih Bozkurt, DVM, PhD. Afyon Kocatepe University Faculty of Veterinary Medicine. Afyonkarahisar - Türkiye +905318506172 fbozkurt@aku.edu.tr fbozkurt@gmail.com www.immunohistokimya.net

Specifications

Product Data

• Components: Pre-Blocking Solution
  Biotinylated Broad Spectrum Secondary Antibody
  HRP-Streptavidin
  DAB Substrate
  DAB Chromogen
• Tissue: Human
• Antibodies: Mouse & Rabbit Antibody
• Chromogen: DAB