SPlink HRP Broad Spectrum DAB Detection Kit

CAT#: D01-18

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Biotin-Streptavidin HRP Detection for MOUSE and RABBIT antibody on human tissue

Size: 6 ml 18 ml 60 ml 110 ml 125 ml

USD 216.00

3 Weeks*

    • 18 ml

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Product Data
Components Pre-Blocking Solution
Biotinylated Broad Spectrum Secondary Antibody
DAB Substrate
DAB Chromogen
Tissue Human
Antibodies Mouse & Rabbit Antibody
Chromogen DAB
Applications IHC


{0} Product Review(s)

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Although this KIT is prepared for human tissues, I have been using it for cat, dog, cow, sheep and buffalo tissues in my laboratory for years. It gives a very clean high signal in both types of tissues.
KIT causes background staining and nonspecific staining in rat and mouse tissues (plasmacytes and sera).
I usually use this KIT with citrate buffer pH6.0 or proteinase K enzyme digestion antegen retrieval methods. Sometimes I also use it with anti-goat antibodies in double staining.
Another important point is that it should be tried to incubate at the temperature and time written in the KIT, especially long strpteavidin-peroxidase (SA-HRP, third reagent) incubation causes background staining. Serum blocking reagent (pre-block) is also very effective.
Here is am adding an image of uroplakin III antibody staining with this KIT.
I hope it helps...

Associate Professor Mehmet Fatih Bozkurt, DVM, PhD.
Afyon Kocatepe University
Faculty of Veterinary Medicine.
Afyonkarahisar - Türkiye
fbozkurt@aku.edu.tr fbozkurt@gmail.com

Mehmet Fatih B. for D01-110 on 04/15/2023

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.