Mouse Icam1 ELISA KIT (96 T)

CAT#: EA800170

Mouse sICAM-1 Immunoassay

Product Images

Representative standard curve for sICAM-1 ELISA

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 4 hours
• Signal: Colorimetric
• Curve Range: 62.5-4000pg/ml
• Sample Type: Serum, plasma, Cell culture supernatant
• Sample Volume: 100 uL
• Specificity: This assay recognizes both natural and recombinant mouse sICAM-1.
• Sensitivity: 30pg/mL
• Reactivities: Mouse
• Interference: No significant interference observed with available related molecules.
• Components:
  • Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate
  • Standard - lyophilized,8000 pg/ml upon reconstitution,2 vials
  • Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials
  • Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial
  • Standard /Sample Diluent - 16 ml/vial,1 bottle
  • Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle
  • Streptavidin-HRP Diluent - 16 ml/vial,1 bottle
  • Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle
  • Substrate Solution - 12 ml/vial,1 bottle
  • Stop Solution - 12 ml/vial,1 bottle
  • Plate Cover Seals,4 pieces
• Background: Intercellular Adhesion Molecule-1 (ICAM-1) is an inducible transmembrane molecule that plays a role in cell migration, antigen presentation and leukocyte activation. Mouse ICAM-1 is an 80-110 kDa, type I transmembrane glycoprotein that is expressed on a variety of cell types. The molecule is 537 amino acids (aa) in length and contains a 27 aa signal sequence, a 458 aa extracellular region, a 24 aa transmembrane segment, and a 28 aa cytoplasmic domain. The extracellular region contains five Ig-like domains and eleven potential N-linked glycosylation sites, many of which are utilized. The first, N-terminal Ig domain (D1) binds LFA-1, while the third domain (D3) binds Mac-1. Notably, glycosylation on the third domain regulates Mac-1 binding. The cytoplasmic domain, while short, is considered to both transduce intracellular signals (via MAP kinase) and interact with the cell cytoskeleton. Membrane ICAM-1 exists as a dimer and will form multimers via D1 interactions. Monomeric ICAM-1 is competent to bind LFA-1. Soluble, dimeric ICAM-1 does circulate and binds LFA-1 with high avidity. Soluble forms are generated via proteolytic processing, reportedly through MMP-9 and elastase. In mice, there are a number of ICAM-1 alternate splice forms that lack combinations of various Ig-domains. This suggests the possibility of multiple truncated forms of proteolytically-generated circulating ICAM-1. Mature mouse ICAM-1 shares 77%, 53%, 56%, and 52% sequence identity to rat, human, canine and porcine ICAM-1, respectively. Cells known to express ICAM-1 include smooth muscle cells, keratinocytes, endothelial cells, fibroblasts, bronchial epithelial cells , memory T cells, B cells, plasma cells, monocytes, macrophages, CFU-E, CFU-GM, activated eosinophils, neutrophils, Schwann cells, Sertoli cells, melanocytes, and dendritic cells.
• Gene Symbol: Icam1
• Gene ID: 15894