Human GDNF ELISA KIT (96 T)

CAT#: EA800083

Human GDNF Immunoassay

Product Images

Representative standard curve for GDNF ELISA

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 4 hours
• Signal: Colorimetric
• Curve Range: 15.62-1000pg/ml
• Sample Type: Serum, plasma, Cell culture supernatant
• Sample Volume: 100 uL
• Specificity: This assay recognizes both natural and recombinant human GDNF.
• Sensitivity: 8pg/mL
• Reactivities: Human
• Interference: No significant interference observed with available related molecules.
• Components:
  • Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate
  • Standard - lyophilized,1000 pg/ml upon reconstitution,2 vials
  • Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials
  • Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial
  • Standard /Sample Diluent - 16 ml/vial,1 bottle
  • Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle
  • Streptavidin-HRP Diluent - 16 ml/vial,1 bottle
  • Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle
  • Substrate Solution - 12 ml/vial,1 bottle
  • Stop Solution - 12 ml/vial,1 bottle
  • Plate Cover Seals,4 pieces
• Background: GDNF (Glial Cell Line-derived Neurotrophic Factor) is a secreted protein that promotes the survival of motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. It is produced by Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. GDNF binding to GFR alpha 1 induces the recruitment of Ret, NCAM-1/CD56, various integrins, Syndecan-3, or N-Cadherin. GDNF-based therapies show promise in several neurodegenerative disorders, particularly Parkinson’s disease.
• Gene Symbol: GDNF
• Gene ID: 2668