Chicken IL6 ELISA KIT (96 T)

CAT#: EA800016

Chicken IL-6 Immunoassay

Product Images

Representative standard curve for IL-6 ELISA

Specifications

Product Data

• Format: 96-well strip plate
• Assay Type: Solid Phase Sandwich ELISA
• Assay Length: 4 hours
• Signal: Colorimetric
• Curve Range: 31.25-2000pg/ml
• Sample Type: Serum, plasma, Cell culture supernatant
• Sample Volume: 100 uL
• Specificity: This assay recognizes both natural and recombinant Chicken IL-6
• Sensitivity: 8 pg/mL
• Reactivities: Chicken
• Interference: No significant interference observed with available related molecules.
• Components:
  • Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate
  • Standard - lyophilized,4000pg/ml upon reconstitution,2 vials
  • lyophilized, Biotin-Conjugated antibody,2 vials
  • Concentrated Streptavidin-HRP,1 vial
  • Standard /sample Diluent,1 bottle
  • Biotin-Conjugate antibody Diluent,1 bottle
  • Streptavidin-HRP Diluent,1 bottle
  • 20 x Wash Buffer Concentrate,1 bottle
  • Substrate Solution,1 bottle
  • Stop Solution,1 bottle
  • Plate Cover Seals,4 pieces
• Background: IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine and is produced by T cells, macrophages, fibroblasts, osteoblasts, endothelial and other cells . IL-6 induces proliferation and differentiation and acts on B cells, T cells, thymocytes, and others. IL-6 is one of the most important mediators of fever and of the acute phase response. In the muscle and fatty tissue, IL-6 stimulates energy mobilization that leads to increased body temperature. IL-6 can be secreted by macrophages in response to specific microbial molecules, referred to as pathogen associated molecular patterns (PAMPS). IL-6 in concert with TGFβ is important for developing Th17 responses. IL-6 binds to IL-6Rα that through association induces gp130 homodimerization . gp130 homodimerization triggers the Jak/STAT cascade and the SHP2/Erk Map kinase cascade . IL-6 also forms a complex with an IL-6Rα splice variant that is non-membrane associated . The IL-6/soluble IL-6Rα complex can then activate the gp130 signaling pathway on cells that express gp130 but not IL6Rα . IL-6 is relevant to many disease processes such as diabetes , atherosclerosis , depression (8), Alzheimer's Disease , systemic lupus erythematosus , prostate cancer , breast cancer (12), and rheumatoid arthritis .
• Gene Symbol: IL6
• Gene ID: 395337