Chicken IL2 ELISA KIT (96 T)

CAT#: EA800012

Chicken IL-2 ELISA Kit


Size
    • 96 T

Product Images

Specifications

Product Data
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 4 hours
Signal Colorimetric
Curve Range 93.75-6000 pg/ml
Sample Type Serum, plasma, Cell culture supernatant
Sample Volume 100 uL
Specificity This assay recognizes both natural and recombinant Chicken IL-2
Sensitivity 23 pg/mL
Reactivities Chicken
Interference No significant interference observed with available related molecules.
Components
  • Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate
  • Standard - lyophilized,6000pg/ml upon reconstitution,2 vials
  • lyophilized, Biotin-Conjugated antibody,2 vials
  • Concentrated Streptavidin-HRP,1 vial
  • Standard /sample Diluent,1 bottle
  • Biotin-Conjugate antibody Diluent,1 bottle
  • Streptavidin-HRP Diluent,1 bottle
  • 20 x Wash Buffer Concentrate,1 bottle
  • Substrate Solution,1 bottle
  • Stop Solution,1 bottle
  • Plate Cover Seals,4 pieces
Background IL-2 is an interleukin, a type of cytokine immune system signaling molecule. IL-2 is a T cell stimulatory cytokine best known for inducing T cell proliferation, the production of immunoglobulins made by B cells and differentiation and proliferation of natural killer cells . IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs) . Conversely, IL-2 is involved in activation induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells . IL-2 signals through a trimeric receptor complex consisting of IL-2 receptor alpha (CD25), IL-2 receptor beta (CD122) and a common gamma chain (γc). IL-2 Rα binds exclusively to IL-2 with low affinity and increases binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-2Rβ is also used by IL-2. The common γc is shared by all members of this family of cytokines including IL-4, IL-7, IL-9, IL-2, and IL-21. Binding of IL-2 activates the Ras/MAPK, JAK/Stat and PI 3-kinase/Akt signaling modules .
Gene Symbol IL2
Gene ID 373958

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