Human BTK ELISA Kit 1 x 96

CAT#: EA200052

Human BTK ELISA Kit 1 x 96

Product Images

Human BTK ELISA Kit.

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Sandwich
• Assay Length: 3.5 hours incubations; 0.5 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 31-5000pg/ml
• Sample Type: Human serum, plasma, cell lysates, tissue homogenates and other biological fluids.
• Sample Volume: 100µl
• Specificity: This kit is used for quantitative detection of BTK
• Sensitivity: 24pg/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • BTK Monoclonal Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate
  • Human BTK Standard (250ng/ml)|0.1 mL
  • 100x Biotin conjugated BTK Detection Antibody|0.12 mL
  • 100x SA-HRP Conjugate|0.12 mL
  • Assay Buffer|30 mL
  • Standard Diluent|10 mL
  • Sample Diluent|30 mL
  • Wash Buffer Concentrate 20X|60 mL
  • TMB Substrate|12 mL
  • Stop Solution|12 mL
  • Plate Sealer|3 pieces
• Background: Bruton's tyrosine kinase (BTK), also known as tyrosine-protein kinase BTK, is a tyrosine kinase that is encoded by the BTK gene in humans. BTK is a 75 kDa cytoplasmic protein tyrosine kinase that is widely expressed in hematopoietic cells. BTK contains five different protein interaction domains. These domains include an amino terminal pleckstrin homology (PH) domain, a proline-rich TEC homology (TH) domain, SRC homology (SH) domains SH2 and SH3, as well as a kinase domain with enzymatic activity. BTK is an essential component of multiple signaling pathways that regulate B cell and myeloid cell proliferation, survival, and functions. Defects in BTK result in X-linked agammaglobulinemia which is characterized by a severely decreased level of circulating antibodies. BTK plays a significant role in the pathogenesis of inflammatory diseases, especially autoimmune diseases, and has been found to regulate cell proliferation, survival, and migration in various B-cell malignancies. Its overexpression and hyperactivation have been reported in acute lymphoblastic leukemias and plasmacytomas, chronic lymphocytic leukemia (CLL)/small lymphocytic lymphoma (SLL), mantle cell lymphoma (MCL), Waldenström’s Macroglobulinemia (WM), Marginal Zone Lymphoma (MZL), in the most common form of non-Hodgkin lymphoma, and in multiple myeloma (MM).
• Gene Symbol: BTK
• Gene ID: 695
• Standard Curve:
  
  Human BTK ELISA Kit.